BMRB Entry 27635

Title:
Microsecond Protein Dynamics from Combined Bloch-McConnell and Near-Rotary-Resonance R1rho Relaxation-Dispersion MAS NMR
Deposition date:
2018-10-03
Original release date:
2019-01-08
Authors:
Marion, Dominique; Gauto, Diego; Ayala, Isabel; Hessel, Audrey; Schanda, Paul
Citation:

Citation: Marion, Dominique; Gauto, Diego; Ayala, Isabel; Giandoreggio-Barranco, Karine; Schanda, Paul. "Microsecond Protein Dynamics from Combined Bloch-McConnell and Near-Rotary-Resonance R1rho Relaxation-Dispersion MAS NMR"  Chemphyschem 20, 276-284 (2019).
PubMed: 30444575

Assembly members:

Assembly members:
Ubiquitin, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Data typeCount
T1rho relaxation values1539

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubiquitin1

Entities:

Entity 1, Ubiquitin 76 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: Ubiquitin, [U-100% 15N], NA mM

sample_conditions_1: temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing

Gamma, Scott A. Smith - Spin evolution simulation

NMR spectrometers:

  • Agilent INOVA 600 MHz