BMRB Entry 27639

Title:
C55 13C Chemical Shifts
Deposition date:
2018-10-07
Original release date:
2019-02-12
Authors:
Smith, Steven; Tang, Tzu-Chun; Hu, Yi; Kienlen-Campard, Pascal; Constantinescu, Stefan; Octave, Jean-Noel; Perrin, Florian; Opsomer, Remi
Citation:

Citation: Tang, Tzu-Chun; Kienlen-Campard, Pascal; Hu, Yi; Perrin, Florian; Opsomer, Remi; Octave, Jean-Noel; Constantinescu, Stefan; Smith, Steven. "Influence of the familial Alzheimer's disease-associated T43I mutation on the transmembrane structure and gamma-secretase processing of the C99 peptide"  J. Biol. Chem. 294, 5854-5866 (2019).
PubMed: 30755484

Assembly members:

Assembly members:
C55, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts21

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Single polypeptide chain C551

Entities:

Entity 1, Single polypeptide chain C55 55 residues - Formula weight is not available

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL
5   ILEALATHRVALILEVALILETHRLEUVAL
6   METLEULYSLYSLYS

Samples:

sample_1: C55, [U-13C], 50 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 7.2; pressure: 1 atm; temperature: 210 K

Experiments:

NameSampleSample stateSample conditions
3D HCACOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz