BMRB Entry 27833

Title:
tEIN backbone and I/L/V methyl resonance assignment
Deposition date:
2019-03-11
Original release date:
2019-05-22
Authors:
Dotas, Rochelle; Venditti, Vincenzo
Citation:

Citation: Dotas, Rochelle; Venditti, Vincenzo. "Resonance assignment of the 128 kDa enzyme I dimer from Thermoanaerobacter tengcongensis"  Biomol. NMR Assignments 13, 287-293 (2019).
PubMed: 31025174

Assembly members:

Assembly members:
tEIN, polymer, 248 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Caldanaerobacter subterraneus   Taxonomy ID: not available   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermoanaerobacter tengcongensis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21A

Data sets:
Data typeCount
13C chemical shifts807
15N chemical shifts229
1H chemical shifts553

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tEIN1

Entities:

Entity 1, tEIN 248 residues - Formula weight is not available

1   METLEULYSGLYVALALAALASERPROGLY
2   ILEALAILEGLYLYSALAPHELEUTYRTHR
3   LYSGLULYSVALTHRILEASNVALGLULYS
4   ILEGLUGLUSERLYSVALGLUGLUGLUILE
5   ALALYSPHEARGLYSALALEUGLUVALTHR
6   GLNGLUGLUILEGLULYSILELYSGLULYS
7   ALALEULYSGLUPHEGLYLYSGLULYSALA
8   GLUILEPHEGLUALAHISLEUMETLEUALA
9   SERASPPROGLULEUILEGLUGLYVALGLU
10   ASNMETILELYSTHRGLULEUVALTHRALA
11   ASPASNALAVALASNLYSVALILEGLUGLN
12   ASNALASERVALMETGLUSERLEUASNASP
13   GLUTYRLEULYSGLUARGALAVALASPLEU
14   ARGASPVALGLYASNARGILEILEGLUASN
15   LEULEUGLYVALLYSSERVALASNLEUSER
16   ASPLEUGLUGLUGLUVALVALVALILEALA
17   ARGASPLEUTHRPROSERASPTHRALATHR
18   METLYSLYSGLUMETVALLEUGLYPHEALA
19   THRASPVALGLYGLYILETHRSERHISTHR
20   ALAILEMETALAARGSERLEUGLUILEPRO
21   ALAVALVALGLYLEUGLYASNVALTHRSER
22   GLNVALLYSALAGLYASPLEUVALILEVAL
23   ASPGLYLEUGLUGLYILEVALILEVALASN
24   PROASPGLULYSTHRVALGLUASPTYRLYS
25   SERLYSLYSGLUSERTYRGLULYS

Samples:

sample_1: tEIN, [U-13C; U-15N; U-2H], 1 mM; D2O 10%; TRIS 20 mM; EDTA 1 mM; sodium chloride 100 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks