BMRB Entry 27837

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for RepA
Deposition date:
2019-03-15
Original release date:
2019-12-17
Authors:
Pantoja-Uceda, David; Oroz, Javier; Laurents, Douglas
Citation:

Citation: Pantoja-Uceda, David; Oroz, Javier; Fernandez, Cristina; de Alba, Eva; Giraldo, Rafael; Laurents, Douglas. "Conformational Priming of RepA-WH1 for Functional Amyloid Conversion Detected by NMR Spectroscopy"  Structure 28, 336-347 (2020).
PubMed: 31918960

Assembly members:

Assembly members:
RepA, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRG-H6-WH1

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts126
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RepA chain 11
2RepA chain 21

Entities:

Entity 1, RepA chain 1 132 residues - Formula weight is not available

1   METVALGLUASNGLNVALTHRGLNSERASN
2   LYSLEUILEGLUSERSERHISTHRLEUTHR
3   LEUASNGLULYSARGLEUVALLEUCYSALA
4   ALASERLEUILEASPSERARGLYSPROLEU
5   PROLYSASPGLYTYRLEUTHRILEARGALA
6   ASPTHRPHEALAGLUVALPHEGLYILEASP
7   VALLYSHISALATYRALAALALEUASPASP
8   ALAALATHRLYSLEUPHEASNARGASPILE
9   ARGARGTYRVALLYSGLYLYSVALVALGLU
10   ARGMETARGTRPVALPHEHISVALLYSTYR
11   ARGGLUGLYGLNGLYCYSVALGLULEUGLY
12   PHESERPROTHRILEILEPROHISLEUTHR
13   METLEUHISLYSGLUPHETHRSERTYRGLN
14   LEULYS

Samples:

sample_1: RepA, [U-99% 13C; U-99% 15N], 1.0 mM; deuterated acetic acid 1.0 mM; mQ 85%; D2O 15%; DSS 50 uM

sample_conditions_1: ionic strength: 0.001 M; pH: 4.0; pressure: 1 atm; temperature: 323.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCO trosysample_1isotropicsample_conditions_1
3D HN(CA)CO trosysample_1isotropicsample_conditions_1
3D HNCA trosysample_1isotropicsample_conditions_1
3D HN(CO)CA trosysample_1isotropicsample_conditions_1
3D CBCACONH trosysample_1isotropicsample_conditions_1
3D HNCACB trosysample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.0, Johnson, One Moon Scientific - data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks