BMRB Entry 27923
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27923
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Citation: Zheng, Yumei; Qiu, Yu; Grace, Christy; Liu, Xu; Klionsky, Daniel; Schulman, Brenda. "A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade" Nat. Commun. 10, 3600-3600 (2019).
PubMed: 31399562
Assembly members:
scAtg3FR(86-159), polymer, 76 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae Vector: pGEX-4T-1
Entity Sequences (FASTA):
scAtg3FR(86-159): GSVEGPDVIMKGFAEDGDED
DVLEYIGSETEHVQSTPAGG
TKDSSIDDIDELIQDMEIKE
EDENDDTEEFNAKGGL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 205 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 71 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | scAtg3FR(86-159) | 1 |
Entities:
Entity 1, scAtg3FR(86-159) 76 residues - Formula weight is not available
| 1 | GLY | SER | VAL | GLU | GLY | PRO | ASP | VAL | ILE | MET | ||||
| 2 | LYS | GLY | PHE | ALA | GLU | ASP | GLY | ASP | GLU | ASP | ||||
| 3 | ASP | VAL | LEU | GLU | TYR | ILE | GLY | SER | GLU | THR | ||||
| 4 | GLU | HIS | VAL | GLN | SER | THR | PRO | ALA | GLY | GLY | ||||
| 5 | THR | LYS | ASP | SER | SER | ILE | ASP | ASP | ILE | ASP | ||||
| 6 | GLU | LEU | ILE | GLN | ASP | MET | GLU | ILE | LYS | GLU | ||||
| 7 | GLU | ASP | GLU | ASN | ASP | ASP | THR | GLU | GLU | PHE | ||||
| 8 | ASN | ALA | LYS | GLY | GLY | LEU |
Samples:
sample_1: scAtg3FR(86-159), [U-100% 13C; U-100% 15N], 0.75 mM; MES 20 mM; sodium chloride 100 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks