BMRB Entry 27928

Title:
RTNLB13_APH_22
Deposition date:
2019-05-28
Original release date:
2020-03-05
Authors:
Brooks, Rhiannon; Dixon, Ann
Citation:

Citation: Brooks, Rhiannon; Dixon, Ann. "Revealing the mechanism of protein-lipid interactions for a putative membrane curvature sensor in plant endoplasmic reticulum"  Biochim. Biophys. Acta Biomembr. 1862, 183160-183160 (2020).
PubMed: 31874147

Assembly members:

Assembly members:
RTNLB13_APH_22, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RTNLB13_APH_22: VPKLWEEYGDQIQKHLGSLK DK

Data sets:
Data typeCount
1H chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R13-APH221

Entities:

Entity 1, R13-APH22 22 residues - Formula weight is not available

1   VALPROLYSLEUTRPGLUGLUTYRGLYASP
2   GLNILEGLNLYSHISLEUGLYSERLEULYS
3   ASPLYS

Samples:

sample_1: RTNLB13 APH 22 1.2 mM; DPC-d38 50 mM; potassium phosphate 25 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CCPNmr_Analysis v2.4, CCPN - chemical shift assignment

TOPSPIN v3.2, Bruker BioSpin - collection, processing

NMR spectrometers:

  • Bruker Avance 700 MHz