BMRB Entry 27973

Title:
Solid-state NMR Chemical Shift Assignments for p150Glued(1-191) of Dynactin
Deposition date:
2019-07-12
Original release date:
2019-08-15
Authors:
Guo, Changmiao; Polenova, Tatyana; Williams, John
Citation:

Citation: Guo, Changmiao; Williams, John; Polenova, Tatyana. "Conformational Flexibility of p150Glued(1-191) Subunit of Dynactin Assembled with Microtubules"  Biophys. J. 117, 938-949 (2019).
PubMed: 31445682

Assembly members:

Assembly members:
p150Glued(1-191), polymer, 191 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Data typeCount
13C chemical shifts296

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p150Glued(1-191) Subunit of Dynactin1

Entities:

Entity 1, p150Glued(1-191) Subunit of Dynactin 191 residues - Formula weight is not available

1   METALAGLNSERLYSARGHISMETTYRASN
2   ARGTHRPROSERGLYSERARGMETSERTHR
3   GLUALASERALAARGPROLEUARGVALGLY
4   SERARGVALGLUVALILEGLYLYSGLYHIS
5   ARGGLYTHRVALALATYRVALGLYALATHR
6   LEUPHEALATHRGLYLYSTRPVALGLYVAL
7   ILELEUASPGLUALALYSGLYLYSASNASP
8   GLYTHRVALGLNGLYARGLYSTYRPHETHR
9   CYSASPGLUGLYHISGLYILEPHEVALARG
10   GLNSERGLNILEGLNVALPHEGLUASPGLY
11   ALAASPTHRTHRSERPROGLUTHRPROASP
12   SERSERALASERLYSILELEULYSARGGLU
13   GLYALAASPALAALAALALYSTHRSERLYS
14   LEUARGGLYLEULYSPROLYSLYSALAPRO
15   THRALAARGLYSTHRTHRTHRARGARGPRO
16   LYSPROTHRARGPROALASERTHRGLYVAL
17   ALAGLYPROSERSERSERLEUGLYPROSER
18   GLYSERALASERALAGLYGLULEUSERSER
19   SERGLUPROSERTHRPROALAGLNTHRPRO
20   LEU

Samples:

sample_complexes: p150Glued(1-191), [U-100% 13C; U-100% 15N], 450 uM; microtubules 540 uM; PIPES 80 mM; MgCl2 1 mM; EGTA 1 mM

sample_microtubules: microtubules 540 uM; PIPES 80 mM; MgCl2 1 mM; EGTA 1 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

sample_conditions_2: pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C CORDsample_complexesisotropicsample_conditions_1
2D NCAsample_complexesisotropicsample_conditions_1
2D NCOsample_complexesisotropicsample_conditions_1
1H-31P CPMASsample_microtubulesisotropicsample_conditions_2
31P direct polarization MAS NMRsample_microtubulesisotropicsample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR, Varian - collection

NMR spectrometers:

  • Varian Uniform NMR System 600 MHz