BMRB Entry 28033

Title:
Backbone assignments for the Asciz QT2-4/LC8 complex
Deposition date:
2019-10-24
Original release date:
2020-03-05
Authors:
Reardon, Patrick; Barbar, Elisar; Jara, Kayla; Smith, Delaney
Citation:

Citation: Reardon, Patrick; Jara, Kayla; Rolland, Amber; Smith, Delaney; Hoang, Hanh; Prell, James; Barbar, Elisar. "The dynein light chain 8 (LC8) binds predominantly "in-register" to a multivalent intrinsically disordered partner"  J. Biol. Chem. 295, 4912-4922 (2020).
PubMed: 32139510

Assembly members:

Assembly members:
QT2-4, polymer, 76 residues, Formula weight is not available
LC8, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET2Zt2-1a

Data sets:
Data typeCount
13C chemical shifts149
15N chemical shifts53
1H chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1QT2-4, chain 11
2QT2-4, chain 21
3LC8, chain 1-12
4LC8, chain 1-22
5LC8, chain 2-12
6LC8, chain 2-22
7LC8, chain 3-12
8LC8, chain 4-22

Entities:

Entity 1, QT2-4, chain 1 76 residues - Formula weight is not available

1   GLYALAMETHISMETASNGLUVALLEUALA
2   PROLEULEUARGASPILEGLUTHRGLNTHR
3   PROASPTHRARGGLYASPILEGLYTHRMET
4   THRASPASPPHEPROGLUGLUGLNGLUPRO
5   VALALAVALGLYSERHISPHEHISALATYR
6   SERGLUTHRGLUPROMETPHEASPLEUGLN
7   THRSERALAHISMETTYRTHRGLNTHRCYS
8   ASPASPLEUPHEGLUGLU

Entity 2, LC8, chain 1-1 92 residues - Formula weight is not available

1   GLYALAHISMETSERASPARGLYSALAVAL
2   ILELYSASNALAASPMETSERGLUGLUMET
3   GLNGLNASPALAVALASPCYSALATHRGLN
4   ALALEUGLULYSTYRASNILEGLULYSASP
5   ILEALAALATYRILELYSLYSGLUPHEASP
6   LYSLYSTYRASNPROTHRTRPHISCYSILE
7   VALGLYARGASNPHEGLYSERTYRVALTHR
8   HISGLUTHRARGHISPHEILETYRPHETYR
9   LEUGLYGLNVALALAILELEULEUPHELYS
10   SERGLY

Samples:

sample_1: QT2-4, [U-13C; U-15N; U-2H], 0.5 mM; LC8 2.0 mM; beta-mercaptoethanol 10 mM; sodium phosphate 10 mM; sodium chloride 10 mM; sodium azide 1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks