BMRB Entry 30507

Title:
Cytokine-like Peptide Stress-response Peptide-1 from Manduca Sexta
Deposition date:
2018-08-13
Original release date:
2019-08-16
Authors:
Schrag, L.
Citation:

Citation: Schrag, L.; Cao, X.; Al Souhail, Q.; Kanost, M.; Jiang, H.; Prakash, O.. "NMR Solution Structure and Expression Profile of Stress Response Peptide-1: A Cytokine from Manduca sexta"  To be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 25 residues, 2818.129 Da.

Natural source:

Natural source:   Common Name: tobacco hornworm   Taxonomy ID: 7130   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Manduca sexta

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: FGVRVGTCPSGYVRRGTFCF PDDDY

Data sets:
Data typeCount
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 25 residues - 2818.129 Da.

1   PHEGLYVALARGVALGLYTHRCYSPROSER
2   GLYTYRVALARGARGGLYTHRPHECYSPHE
3   PROASPASPASPTYR

Samples:

sample_1: Stress-response Peptide-1 1.8 ± 0.1 mM

sample_conditions_1: pH: 2.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D DQF-COSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1

Software:

VNMR v3.2a, Varian - collection, processing

CcpNMR v2.4, CCPN - peak picking

Analysis v2.4, CCPN - chemical shift assignment

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

ProcheckNMR, Laskowski and MacArthur - refinement

NMR spectrometers:

  • Varian UNITY 500 MHz