BMRB Entry 30566

Title:
hGRNA4-28_3s
Deposition date:
2019-02-01
Original release date:
2020-01-30
Authors:
Dastpeyman, M.; Bansal, P.; Wilson, D.; Loukas, A.; Smout, M.; Daly, N.
Citation:

Citation: Dastpeyman, M.; Bansal, P.; Wilson, D.; Sotillo, J.; Brindley, P.; Loukas, A.; Smout, M.; Daly, N.. "hGRN4-28_3s"  to be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 24 residues, 2588.959 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: CDMEVSCPDGYTCCRLQSGA WGCC

Data sets:
Data typeCount
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 24 residues - 2588.959 Da.

1   CYSASPMETGLUVALSERCYSPROASPGLY
2   TYRTHRCYSCYSARGLEUGLNSERGLYALA
3   TRPGLYCYSCYS

Samples:

sample_1: hGRNA4-28_3s 0.5 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1

Software:

TOPSPIN v3.5PL6, Bruker Biospin - collection

CYANA v3.97, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

CcpNMR, CCPN - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz