BMRB Entry 30652

Name: Human CstF-64 RRM
Published: 2020-08-03

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PDB ID: 6q2i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30652
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human CstF-64 RRM

Deposition date:

2019-08-08

Original release date:

2020-08-03

Authors:

Latham, M.; Masoumzadeh, E.

Citation:

Citation: Grozdanov, Petar; Masoumzadeh, Elahe; Kalscheuer, Vera; Bienvenu, Thierry; Billuart, Pierre; Delrue, Marie-Ange; Latham, Michael; MacDonald, Clinton. "A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans" Nucleic Acids Res. 48, 9804-9821 (2020).
PubMed: 32816001

Assembly members:

Assembly members:
entity_1, polymer, 108 residues, 11965.345 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAGLTVRDPAVDRSLRSVFV GNIPYEATEEQLKDIFSEVG PVVSFRLVYDRETGKPKGYG FCEYQDQETALSAMRNLNGR EFSGRALRVDNAASEKNKEE LKSLGTGA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	241
15N chemical shifts	102
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 108 residues - 11965.345 Da.

1

MET

ALA

GLY

LEU

THR

VAL

ARG

ASP

PRO

ALA

2

VAL

ASP

ARG

SER

LEU

ARG

SER

VAL

PHE

VAL

3

GLY

ASN

ILE

PRO

TYR

GLU

ALA

THR

GLU

4

GLN

LEU

LYS

ASP

ILE

PHE

SER

GLU

VAL

GLY

5

PRO

VAL

SER

PHE

ARG

LEU

VAL

TYR

ASP

6

ARG

GLU

THR

GLY

LYS

PRO

LYS

GLY

TYR

GLY

7

PHE

CYS

GLU

TYR

GLN

ASP

GLN

GLU

THR

ALA

8

LEU

SER

ALA

MET

ARG

ASN

LEU

ASN

GLY

ARG

9

GLU

PHE

SER

GLY

ARG

ALA

LEU

ARG

VAL

ASP

10

ASN

ALA

SER

GLU

LYS

ASN

LYS

GLU

11

LEU

LYS

SER

LEU

GLY

THR

GLY

ALA

Samples:

sample_1: RNA Recognition Motif, [U-100% 13C; U-100% 15N], 980 uM

sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment

NMRDraw v9.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, peak picking

CS-ROSETTA, Shen, Vernon, Baker and Bax - refinement, structure calculation

NMR spectrometers:

Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks