BMRB Entry 34253

Title:
The solution NMR structure of [C18S,C24S]brevinin-1BYa in 33% trifluoroethanol
Deposition date:
2018-03-28
Original release date:
2019-10-10
Authors:
Timmons, P.; O'Flynn, D.; Conlon, J.; Hewage, C.
Citation:

Citation: Timmons, P.; O'Flynn, D.; Conlon, J.; Hewage, C.. "Insights into conformation and membrane interactions of the acyclic and dicarba-bridged brevinin-1BYa antimicrobial peptides."  Eur. Biophys. J. 48, 701-710 (2019).
PubMed: 31515575

Assembly members:

Assembly members:
entity_1, polymer, 24 residues, 2581.185 Da.

Natural source:

Natural source:   Common Name: Foothill yellow-legged frog   Taxonomy ID: 160499   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana boylii

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: FLPILASLAAKFGPKLFSLV TKKS

Data sets:
Data typeCount
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 24 residues - 2581.185 Da.

1   PHELEUPROILELEUALASERLEUALAALA
2   LYSPHEGLYPROLYSLEUPHESERLEUVAL
3   THRLYSLYSSER

Samples:

sample_1: [C18S,C24S]brevinin-1BYa 1.5 mM

sample_conditions_1: pH: 7; pressure: 100000 Pa; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert P. - structure calculation

NMRFAM-Sparky, NATIONAL MAGNETIC RESONANCE FACILITY AT MADISON - peak picking

NMR spectrometers:

  • Bruker DRX 500 MHz