BMRB Entry 34380

Title:
Structure of peptide P7, which binds Cdc42 and inhibits effector interactions.
Deposition date:
2019-03-15
Original release date:
2020-01-24
Authors:
Murphy, N.; Mott, H.; Owen, D.
Citation:

Citation: Tetley, G.; Murphy, N.; Bonetto, S.; Ivanova, G.; Revell, J.; Mott, H.; Cooley, R.; Owen, D.. "The discovery and maturation of peptide biologics targeting the small G protein Cdc42: a bioblockade for Ras-driven signalling"  J. Biol. Chem. 295, 2866-2884 (2020).
PubMed: 31959628

Assembly members:

Assembly members:
entity_1, polymer, 16 residues, 2070.401 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PSIXHVHRPDWPCWYR

Data sets:
Data typeCount
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 16 residues - 2070.401 Da.

1   PROSERILEHCSHISVALHISARGPROASP
2   TRPPROCYSTRPTYRARG

Samples:

sample_1: peptide 5 mg/mL; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsample_1isotropicsample_conditions_1
COSYsample_1isotropicsample_conditions_1
NOESYsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis v2, CCPN - chemical shift assignment

ARIA v1.2, Linge, O'Donoghue and Nilges - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMR spectrometers:

  • Bruker AVANCE III 800 MHz