BMRB Entry 34411

Title:
Winter flounder 1a in SDS micelles
Deposition date:
2019-06-11
Original release date:
2020-07-10
Authors:
Mason, A.; Clarke, M.
Citation:

Citation: Clarke, M.; Mason, A.. "Winter flounder 1a in SDS micelles"  .

Assembly members:

Assembly members:
entity_1, polymer, 20 residues, 2178.647 Da.

Natural source:

Natural source:   Common Name: Winter flounder   Taxonomy ID: 8265   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Pseudopleuronectes americanus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: WLRRIGKGVKIIGGAALDHL

Data sets:
Data typeCount
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 20 residues - 2178.647 Da.

1   TRPLEUARGARGILEGLYLYSGLYVALLYS
2   ILEILEGLYGLYALAALALEUASPHISLEU

Samples:

sample_1: Peptide 0.5 mM; SDS, U-98% SDS D-25, 50 mM; TSP 0.005 % w/w

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_1isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

CARA, Keller and Wuthrich - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

DYNAMO, Delaglio and Kuszewski - refinement, structure calculation

NMR spectrometers:

  • Bruker Avance 700 MHz