BMRB Entry 36072

Title:
C-terminal zinc finger of RING finger protein 141
Deposition date:
2017-04-05
Original release date:
2018-04-30
Authors:
Miyamoto, K.
Citation:

Citation: Miyamoto, Kazuhide; Uechi, Airi; Saito, Kazuki. "The zinc finger domain of RING finger protein 141 reveals a unique RING fold."  Protein Sci. 26, 1681-1686 (2017).
PubMed: 28547869

Assembly members:

Assembly members:
RING finger protein 141, polymer, 42 residues, 4920.788 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING finger protein 141: EEECCICMDGRADLILPCAH SFCQKCIDKWSDRHRNCPIC RL

Data sets:
Data typeCount
1H chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_ZN_12
3entity_ZN_22

Entities:

Entity 1, entity_1 42 residues - 4920.788 Da.

1   GLUGLUGLUCYSCYSILECYSMETASPGLY
2   ARGALAASPLEUILELEUPROCYSALAHIS
3   SERPHECYSGLNLYSCYSILEASPLYSTRP
4   SERASPARGHISARGASNCYSPROILECYS
5   ARGLEU

Entity 2, entity_ZN_1 - Zn 2 - 65.409 Da.

1   ZN

Samples:

sample_1: RING finger protein 141, [U-13C; U-15N], 1.0 mM; Tris-HCl 20 mM; NaCl 50 mM; dithiothreitol 1 mM; ZnCl2 50 uM; H2O 90%; D2O, U-2H, 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.9; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, peak picking, refinement

Discovery Studio v2.1, Accelrys Software Inc. - structure calculation

NMR spectrometers:

  • Bruker Avance 800 MHz