BMRB Entry 4241

Title:
1H and 15N Assignments of Internal xylan binding domain from XYLD
Deposition date:
1998-10-09
Original release date:
1999-11-29
Authors:
Simpson, Peter; Bolam, David; Cooper, Alan; Ciruela, Antonio; Hazlewood, Geoffrey; Gilbert, Harry; Williamson, Michael
Citation:

Citation: Simpson, Peter; Bolam, David; Cooper, Alan; Ciruela, Antonio; Hazlewood, Geoffrey; Gilbert, Harry; Williamson, Michael. "The Three-Dimensional Structure of a Family IIb Xylan Binding Domain: Evidence that the Orientation of the Solvent-Exposed Tryptophans Determines the Ligand Specificity of Type II Polysaccharide Binding Domains."  Structure 7, 853-864 (1999).

Assembly members:

Assembly members:
xylanase D, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1708   Superkingdom: eubacteria   Kingdom: not available   Genus/species: Cellulomonas fimi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts100
1H chemical shifts532

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1XBD1-XYLD1

Entities:

Entity 1, XBD1-XYLD 87 residues - Formula weight is not available

1   THRGLYCYSSERVALTHRALATHRARGALA
2   GLUGLUTRPSERASPARGPHEASNVALTHR
3   TYRSERVALSERGLYSERSERALATRPTHR
4   VALASNLEUALALEUASNGLYSERGLNTHR
5   ILEGLNALASERTRPASNALAASNVALTHR
6   GLYSERGLYSERTHRARGTHRVALTHRPRO
7   ASNGLYSERGLYASNTHRPHEGLYVALTHR
8   VALMETLYSASNGLYSERSERTHRTHRPRO
9   ALAALATHRCYSALAGLYSER

Samples:

sample_one: xylanase D, [U-99% 15N], 1.0 mM; sodium_phosphate 50 mM

sample_conditions_one: pH: 5.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
COSYsample_onenot availablenot available
NOESYsample_onenot availablenot available
TOCSYsample_onenot availablenot available
E. COSYsample_onenot availablenot available
1H-15N HSQCsample_onenot availablenot available
NOESY-HMQCsample_onenot availablenot available
TOCSY-HMQCsample_onenot availablenot available
HNHAsample_onenot availablenot available
HNHBsample_onenot availablenot available

Software:

FELIX v97.0 -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 4623
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks