BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4609

Title: NMR observation of T-tetrads in a parallel stranded DNA quadruplex formed by Saccharomyces cerevisiae telomere sequence

Authors: Hosur, R.; Patel, P.

Citation: Hosur, R.; Patel, P.. "NMR observation of T-tetrads in a parallel stranded DNA quadruplex formed by Saccharomyces cerevisiae telomere sequence"  Nucleic Acids Res. 27, 2457-2464 (1999).

Assembly members:
yeast telomere repeats, polymer, 7 residues, Formula weight is not available

Natural source:   Common Name: Yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
yeast telomere repeats: TGGTGGC

Data sets:
Data typeCount
1H chemical shifts59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1yeast telomere repeats, strand 1a1
2yeast telomere repeats, strand 1b1
3yeast telomere repeats, strand 2a1
4yeast telomere repeats, strand 2b1

Entities:

Entity 1, yeast telomere repeats, strand 1a 7 residues - Formula weight is not available

1   DTDGDGDTDGDGDC

Samples:

sample_1: yeast telomere repeats1 – 2 mM; potassium phosphate 10 mM; EDTA 0.2 mM; KCl 100 mM; H2O 90%; D2O 10%

sample_2: yeast telomere repeats1 – 2 mM; potassium phosphate 10 mM; EDTA 0.2 mM; KCl 100 mM; D2O 100%

sample_cond_1: pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
E COSYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1

Software:

FELIX v230 - data processing

VNMR v6.1 - data collection

DISCOVER v3.1 - structure solution

IRMA v2.3 - refinement

NMR spectrometers:

  • Bruker AMX 500 MHz

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