BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4614

Title: The G7(syn)-G4(anti) structure of r(GCAGGCGUGC)2

Authors: Burkard, Mark; Turner, Douglas

Citation: Burkard, Mark; Turner, Douglas. "The NMR structures of r(GCAGGCGUGC)2 and determinants of stability for single guanosine-guanosine base pairs"  Biochemistry 39, 11748-11762 (2000).

Assembly members:
5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3', polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3': GCAGGCGUGC

Data sets:
Data typeCount
1H chemical shifts54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GCAGGCGUGC strand 11
2GCAGGCGUGC strand 21

Entities:

Entity 1, GCAGGCGUGC strand 1 10 residues - Formula weight is not available

1   GCAGGCGUGC

Samples:

D2O_sample: 5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3' 3.0 mM; NaCl 80 mM; phosphate 10 mM; D2O 100%

H2O_sample: 5'-r(*GP*CP*AP*GP*GP*CP*GP*UP*GP*C)-3' 1.0 mM; NaCl 80 mM; Na_phosphate 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 90 mEq; pH: 7; temperature: 310.15 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1

Software:

VNMR v5.2 - data collection

FELIX v95.0 - data analysis

DISCOVER v95.0 - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links: