BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4615

Title: Solution Structure of PAFP-S: A new Knottin-type Antifungal Peptide from the seeds of Phytolacca americana   PubMed: 11551192

Authors: Gao, G.; Liu, W.; Dai, J.; Wang, J.; Hu, Z.; Zhang, Y.; Wang, D.

Citation: Gao, G.; Liu, W.; Dai, J.; Wang, J.; Hu, Z.; Zhang, Y.; Wang, D.. "Solution Structure of PAFP-S: A new Knottin-type Antifungal Peptide from the seeds of Phytolacca americana"  Biochemistry 40, 10973-10978 (2001).

Assembly members:
antifungal peptide, polymer, 38 residues, Formula weight is not available

Natural source:   Common Name: Pokeweed   Taxonomy ID: 3527   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Phytolacca americana

Experimental source:   Production method: purified from natural source

Entity Sequences (FASTA):
antifungal peptide: AGCIKNGGRCNASAGPPYCC SSYCFQIAGQSYGVCKNR

Data sets:
Data typeCount
1H chemical shifts203

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1antifungal peptide1

Entities:

Entity 1, antifungal peptide 38 residues - Formula weight is not available

1   ALAGLYCYSILELYSASNGLYGLYARGCYS
2   ASNALASERALAGLYPROPROTYRCYSCYS
3   SERSERTYRCYSPHEGLNILEALAGLYGLN
4   SERTYRGLYVALCYSLYSASNARG

Samples:

sample_1: antifungal peptide 4 mM; DSS 0.2 mM

sample_cond_1: pH: 3.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
2D COSYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

UXNMR v1.0 - COLLECTION

FELIX v97 - PROCESSING

X-PLOR v3.851 - REFINEMENT

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAA88893
GB AAC05129 AAD17942
SP P81418