BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4619

Title: N-terminal zinc-binding HHCC domain of HIV-2 integrase

Authors: Eijkelenboom, A.; van den Ent, F.; Plasterk, R.; Kaptein, R.; Boelens, R.

Citation: Eijkelenboom, A.; van den Ent, F.; Vos, A.; Doreleijers, J.; Hard, K.; Tullius, T.; Plasterk, R.; Kaptein, R.; Boelens, R.. "Solution structure of the N-terminal HHCC domain of HIV-2 integrase: a three-helix bundle stabilized by zinc"  Curr. Biol. 7, 739-746 (1997).

Assembly members:
Human immunodeficiency virus type 2 integrase, polymer, 55 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: HIV-2   Taxonomy ID: 11720   Superkingdom: not available   Kingdom: not available   Genus/species: not available Human immunodeficiency virus type 2

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Human immunodeficiency virus type 2 integrase: FLEKIEPAQEEHEKYHSNVK ELSHKFGIPNLVARQIVNSC AQCQQKGEAIHGQVN

Data sets:
Data typeCount
1H chemical shifts398
15N chemical shifts62
13C chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HHCC domain of HIV-2 monomer 11
2HHCC domain of HIV-2 monomer 21
3Zn-12
4Zn-22

Entities:

Entity 1, HHCC domain of HIV-2 monomer 1 55 residues - Formula weight is not available

1   PHELEUGLULYSILEGLUPROALAGLNGLU
2   GLUHISGLULYSTYRHISSERASNVALLYS
3   GLULEUSERHISLYSPHEGLYILEPROASN
4   LEUVALALAARGGLNILEVALASNSERCYS
5   ALAGLNCYSGLNGLNLYSGLYGLUALAILE
6   HISGLYGLNVALASN

Entity 2, Zn-1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Human immunodeficiency virus type 2 integrase, [U-15N], 3.5 mM; ZINC (II) ION 1.1 eq; Tris, [U-2H], 50 mM; NaCl 150 mM; 2-mercaptoethanol 2 mM; D2O 99.99%

sample_2: Human immunodeficiency virus type 2 integrase, [U-15N], 3.5 mM; ZINC (II) ION 1.1 eq; Tris, [U-2H], 50 mM; NaCl 150 mM; 2-mercaptoethanol 2 mM; H2O 95%; D2O 5%

sample_3: Human immunodeficiency virus type 2 integrase, [U-15N; U-13C], 3.5 mM; ZINC (II) ION 1.1 eq; Tris, [U-2H], 50 mM; NaCl 150 mM; 2-mercaptoethanol 2 mM; D2O 5%

sample_4: Human immunodeficiency virus type 2 integrase, [U-15N; U-13C], 3.5 mM; ZINC (II) ION 1.1 eq; Tris, [U-2H], 50 mM; NaCl 150 mM; 2-mercaptoethanol 2 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D (1H,15N)-HSQCnot availablenot availablesample_cond_1
2D (1H,15N)-HSMQCnot availablenot availablesample_cond_1
2D (1H,1H) NOESYnot availablenot availablesample_cond_1
2D (1H,1H) TOCSYnot availablenot availablesample_cond_1
3D TOCSY (15N,1H) HSQCnot availablenot availablesample_cond_1
3D TOCSY (13C,1H) HSQCnot availablenot availablesample_cond_1
3D NOESY (15N,1H) HSQCnot availablenot availablesample_cond_1
3D NOESY (13C,1H) HSQCnot availablenot availablesample_cond_1
3D (13C) HMQC NOESY (13C,1H) HSQCnot availablenot availablesample_cond_1
2D 13C-filtered NOESYnot availablenot availablesample_cond_1
3D 13C-filtered NOESY (13C,1H) HSQCnot availablenot availablesample_cond_1
3D HNCOnot availablenot availablesample_cond_1
3D HNCAnot availablenot availablesample_cond_1
3D HN(CO)CAnot availablenot availablesample_cond_1
3D HNHAnot availablenot availablesample_cond_1
3D HNHBnot availablenot availablesample_cond_1
3D H(C)CH DIPSInot availablenot availablesample_cond_1
3D HCC(H) DIPSInot availablenot availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity Plus 500 MHz
  • Bruker AMX 600 MHz
  • Varian Unity Plus 750 MHz

Related Database Links:

PDB
EMBL CAA08005 CAA08033 CAA08041
GB AAB00764 ABU88863 ADQ26695 AEI83209 AHI42063
PRF 1306388B
SP P04584