BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4620

Title: Human prion protein variant R220K

Authors: Calzonai, L.; Lysek, D.; Guntert, P.; Von Schroetter, C.; Zahn, R.; Riek, R.; Wuthrich, K.

Citation: Calzonai, L.; Lysek, D.; Guntert, P.; Von Schroetter, C.; Zahn, R.; Riek, R.; Wuthrich, K.. "NMR structures of three single-residue variants of the human prion protein"  Proc. Natl. Acad. Sci. U. S. A. 97, 8340-8345 (2000).

Assembly members:
prion protein, polymer, 112 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
prion protein: GSVVGGLGGYMLGSAMSRPI IHFGSDYEDRYYRENMHRYP NQVYYRPMDEYSNQNNFVHD CVNITIKQHTVTTTTKGENF TETDVKMMERVVEQMCITQY EKESQAYYQRGS

Data sets:
Data typeCount
13C chemical shifts355
1H chemical shifts768
15N chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1prion protein1

Entities:

Entity 1, prion protein 112 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROILE
3   ILEHISPHEGLYSERASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETHISARGTYRPRO
5   ASNGLNVALTYRTYRARGPROMETASPGLU
6   TYRSERASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRILELYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPVALLYSMETMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLULYSGLUSERGLNALATYRTYRGLNARG
12   GLYSER

Samples:

sample_1: prion protein, [U-13C; U-15N], 1.0 mM; sodium acetate 50 mM

sample_cond_1: pH: 4.5; temperature: 293 K; ionic strength: 50 mM

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availablesample_cond_1
HN(CO)CAsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1
15N-resolved (1H,1H)NOESYsample_1not availablesample_cond_1
13C-resolved (1H,1H)NOESYsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

BMRB 16743 16757 17714 17756 17757 17780 18426 18550 19268 4379 4402 4434 4641 4736
PDB
DBJ BAA00011 BAG32276 BAG32277 BAG32278 BAG32279
EMBL CAA58442 CAG46836 CAG46869
GB AAA19664 AAA60182 AAA68633 AAB59442 AAB59443
REF NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902
SP P04156 P40252