BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4622

Title: Solution structure, Hydrodynamics and thermodynamics of the UvrB C-terminal domain   PubMed: 11697728

Authors: Alexandrovich, A.; Czish, M.; Frenkiel, T.; Kelly, G.; Goosen, N.; Moolenaar, G.; Chowdhry, B.; Sanderson, M.; Lane, A.

Citation: Alexandrovich, A.; Czisch, M.; Frenkiel, T.; Kelly, G.; Goosen, N.; Moolenaar, G.; Chowdhry, B.; Sanderson, M.; Lane, A.. "Solution Structure, Hydrodynamics and Thermodynamics of the UvrB C-terminal Domain"  J. Biomol. Struct. Dyn. 19, 219-236 (2001).

Assembly members:
UvrB C-terminal domain, polymer, 63 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UvrB C-terminal domain: MHHHHHHLEPDNVPMDMSPK ALQQKIHELEGLMMQHAQNL EFEEAAQIRDQLHQLRELFI AAS

Data sets:
Data typeCount
13C chemical shifts190
1H chemical shifts374
15N chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UvrB monomer 11
2UvrB monomer 21

Entities:

Entity 1, UvrB monomer 1 63 residues - Formula weight is not available

1   METHISHISHISHISHISHISLEUGLUPRO
2   ASPASNVALPROMETASPMETSERPROLYS
3   ALALEUGLNGLNLYSILEHISGLULEUGLU
4   GLYLEUMETMETGLNHISALAGLNASNLEU
5   GLUPHEGLUGLUALAALAGLNILEARGASP
6   GLNLEUHISGLNLEUARGGLULEUPHEILE
7   ALAALASER

Samples:

sample_1: UvrB C-terminal domain, [U-13C; U-15N], 10 mg/mL

sample_cond_1: pH: 7.5; temperature: 293 K; ionic strength: 0.2 M

Experiments:

NameSampleSample stateSample conditions
15N-edited HSQC-NOESYsample_1not availablesample_cond_1
13C-edited HSQC-NOESYsample_1not availablesample_cond_1
13C/12C-filtered NOESYsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA35437 BAB34280 BAG76357 BAI24221 BAI29665
EMBL CAA27314 CAA27357 CAP75249 CAQ31280 CAQ89833
GB AAC73866 AAG55150 AAN79333 AAZ87507 ABB65345
REF NP_308884 NP_415300 WP_000042508 WP_000042514 WP_000042517
SP A1A921 A7ZJI8 A7ZY35 B1IXI9 B1LM70