BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4628

Title: Heavy Chain Variable domain from LLama

Authors: Renisio, J.-G.; Perez, J.; Czisch, M.; Guenneugues, M.; Bornet, O.; Frenken, L.; Cambillau, C.; Darbon, H.

Citation: Renisio, J.-G.; Perez, J.; Czisch, M.; Guenneugues, M.; Bornet, O.; Frenken, L.; Cambillau, C.; Darbon, H.. "Solution structure and backbone dynamics of an antigen-free heavy chain variable domain (VHH) from Llama"  Proteins: Struct., Funct., Genet. 47, 546-555 (2002).

Assembly members:
H14, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: llama   Taxonomy ID: 9844   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lama glama

Experimental source:   Production method: .

Entity Sequences (FASTA):
H14: QVQLQESGGGLVQAGGSLRL SCAASGRTGSTYDMGWFRQA PGKERESVAAINWDSARTYY ASSVRGRFTISRDNAKKTVY LQMNSLKPEDTAVYTCGAGE GGTWDSWGQGTQVTVSS

Data sets:
Data typeCount
1H chemical shifts679
coupling constants46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H141

Entities:

Entity 1, H14 117 residues - Formula weight is not available

1   GLNVALGLNLEUGLNGLUSERGLYGLYGLY
2   LEUVALGLNALAGLYGLYSERLEUARGLEU
3   SERCYSALAALASERGLYARGTHRGLYSER
4   THRTYRASPMETGLYTRPPHEARGGLNALA
5   PROGLYLYSGLUARGGLUSERVALALAALA
6   ILEASNTRPASPSERALAARGTHRTYRTYR
7   ALASERSERVALARGGLYARGPHETHRILE
8   SERARGASPASNALALYSLYSTHRVALTYR
9   LEUGLNMETASNSERLEULYSPROGLUASP
10   THRALAVALTYRTHRCYSGLYALAGLYGLU
11   GLYGLYTHRTRPASPSERTRPGLYGLNGLY
12   THRGLNVALTHRVALSERSER

Samples:

sample_1: H14, [U-15N], 3 mM; Na acetate, [U-2H], 10 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4.6; temperature: 300 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1

Software:

UXNMR v2.6 - data collection, data processing

xeasy v1.3.13 - data analysis

diana v2.8 - structure solution

cns v1.0 - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
EMBL CAA15406