BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4633

Title: Solution Structure, Backbone Dynamics, and stability of a Double Mutant Single-Chain Monellin. Structural origin of sweetness   PubMed: 11279156

Authors: Sung, Y.; Shin, J.; Chang, H.; Cho, J.; Lee, W.

Citation: Sung, Y.; Shin, J.; Chang, H.; Cho, J.; Lee, W.. "Solution Structure, Backbone Dynamics, and stability of a Double Mutant Single-Chain Monellin. Structural origin of sweetness "  J. Biol. Chem. 276, 19624-19630 (2001).

Assembly members:
MONELLIN, polymer, 91 residues, Formula weight is not available

Natural source:   Common Name: Serendipity berry   Taxonomy ID: 3457   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Dioscoreophyllum cumminsii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MONELLIN: GEWEIIEIGPFYQNLGKFAV DEENKIGQYGRLTFNKVIKP CMKKTIYENEREIKGYEYQL YVYASDKLFRADISEDYKTR GRKLLRFNGPV

Data sets:
Data typeCount
1H chemical shifts596

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MONELLIN1

Entities:

Entity 1, MONELLIN 91 residues - Formula weight is not available

1   GLYGLUTRPGLUILEILEGLUILEGLYPRO
2   PHETYRGLNASNLEUGLYLYSPHEALAVAL
3   ASPGLUGLUASNLYSILEGLYGLNTYRGLY
4   ARGLEUTHRPHEASNLYSVALILELYSPRO
5   CYSMETLYSLYSTHRILETYRGLUASNGLU
6   ARGGLUILELYSGLYTYRGLUTYRGLNLEU
7   TYRVALTYRALASERASPLYSLEUPHEARG
8   ALAASPILESERGLUASPTYRLYSTHRARG
9   GLYARGLYSLEULEUARGPHEASNGLYPRO
10   VAL

Samples:

sample_1: MONELLIN 2 mM; photassium phosphate 50 mM; H20 90%; D20 10%

sample_2: MONELLIN, [U-15N], 2 mM; photassium phosphate 50 mM; H20 90%; D20 10%

sample_3: MONELLIN 2 mM; photassium phosphate 50 mM; D20 100%

sample_4: MONELLIN, [U-15N], 2 mM; photassium phosphate 50 mM; D20 100%

sample_cond_1: pH: 7.0; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
HSQC(exchange)not availablenot availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz

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