BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4634

Title: Structural analysis of multi-functional peptide motifs present in human bifunctional tRNA synthetase: Identification of RNA-binding residues and functional implications for tandem repeats   PubMed: 11123902

Authors: Jeong, E.-J.; Hwang, G.-S.; Kim, K.; Kim, M.; Kim, S.; Kim, K.-S.

Citation: Jeong, E.-J.; Hwang, G.-S.; Kim, K.; Kim, M.; Kim, S.; Kim, K.-S.. "Structural analysis of multi-functional peptide motifs present in human bifunctional tRNA synthetase: Identification of RNA-binding residues and functional implications for tandem repeats"  Biochemistry 39, 15775-15782 (2000).

Assembly members:
Multifunctional aminoacyl-tRNA synthetase(E.C.6.1.1.17), polymer, 57 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Multifunctional aminoacyl-tRNA synthetase(E.C.6.1.1.17): DSLVLYNRVAVQGDVVRELK AKKAPKEDVDAAVKQLLSLK AEYKEKTGQEYKPGNPP

Data sets:
Data typeCount
coupling constants46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Multifunctional aminoacyl-tRNA synthetase1

Entities:

Entity 1, Multifunctional aminoacyl-tRNA synthetase 57 residues - Formula weight is not available

1   ASPSERLEUVALLEUTYRASNARGVALALA
2   VALGLNGLYASPVALVALARGGLULEULYS
3   ALALYSLYSALAPROLYSGLUASPVALASP
4   ALAALAVALLYSGLNLEULEUSERLEULYS
5   ALAGLUTYRLYSGLULYSTHRGLYGLNGLU
6   TYRLYSPROGLYASNPROPRO

Samples:

sample_1: Multifunctional aminoacyl-tRNA synthetase(E.C.6.1.1.17), [U-15N], 2 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1

Software:

NMRPipe v1.7 - processing

PIPP v4.2.5 - data analysis

X-PLOR v3.851 - structure solution

NMR spectrometers:

  • Varian UNITY 600 MHz

Related Database Links:

PDB