BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4637

Title: SOLUTION STRUCTURE OF THE MATA1 HOMEODOMAIN   PubMed: 10955992

Authors: Anderson, J.; Forman, M.; Modleski, S.; Dahlquist, F.; Baxter, S.

Citation: Anderson, J.; Forman, M.; Modleski, S.; Dahlquist, F.; Baxter, S.. "Cooperative ordering in homeodomain-DNA recognition: solution structure and dynamics of the MATa1 homeodomain"  Biochemistry 39, 10045-10054 (2000).

Assembly members:
MATING-TYPE PROTEIN A-1, polymer, 61 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MATING-TYPE PROTEIN A-1: KKEKSPKGKSSISPQARAFL EQVFRRKQSLNSKEKEEVAK KCGITPLQVRVWFINKRMRS K

Data sets:
Data typeCount
1H chemical shifts353
13C chemical shifts119
15N chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MATING-TYPE PROTEIN A-11

Entities:

Entity 1, MATING-TYPE PROTEIN A-1 61 residues - Formula weight is not available

1   LYSLYSGLULYSSERPROLYSGLYLYSSER
2   SERILESERPROGLNALAARGALAPHELEU
3   GLUGLNVALPHEARGARGLYSGLNSERLEU
4   ASNSERLYSGLULYSGLUGLUVALALALYS
5   LYSCYSGLYILETHRPROLEUGLNVALARG
6   VALTRPPHEILEASNLYSARGMETARGSER
7   LYS

Samples:

sample_1: MATING-TYPE PROTEIN A-1, [U-15N; U-13C], 1.5 mM; acetate, [U-2H], 25 mM; KCl 100 mM; NaN3 0.01%; H2O 90%; D2O 10%

sample_2: MATING-TYPE PROTEIN A-1, [U-15N], 1.5 mM; acetate, [U-2H], 25 mM; KCl 100 mM; NaN3 0.01%; H2O 90%; D2O 10%

sample_3: MATING-TYPE PROTEIN A-1 1.5 mM; acetate, [U-2H], 25 mM; KCl 100 mM; NaN3 0.01%; H2O 90%; D2O 10%

sample_cond_1: pH: 4.5; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
HNCA-Jnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1

Software:

XWINNMR v2.3 - collection

FELIX v970 - data analysis, processing

X-PLOR v3.851 - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ GAA21999
EMBL CAA42252 CAY78246 CAY78300
GB AHY79765 AJP37516 AJQ32001 AJQ32146 AJQ32291
REF NP_010021
SP P0CY10 P0CY11
TPG DAA07567