BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4638

Title: SOLUTION STRUCTURE OF MNEI, A SWEET PROTEIN   PubMed: 11152608

Authors: Temussi, P.; Spadaccini, R.

Citation: Spadaccini, R.; Crescenzi, O.; tancredi, T.; De casamassimi, N.; saviano, G.; scognamiglio, R.; Di Donato, A.; Temussi, P.. "Solution structure of a sweet protein: NMR study of MNEI, a single chain monellin"  J. Mol. Biol. 305, 505-514 (2001).

Assembly members:
MNEI SWEET PROTEIN RELATED TO MONELLIN, polymer, 96 residues, Formula weight is not available

Natural source:   Common Name: serendipity berry   Taxonomy ID: 3457   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Discoreophyllum cuminsii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MNEI SWEET PROTEIN RELATED TO MONELLIN: GEWEIIDIGPFTQNLGKFAV DEENKIGQYGRLTFNKVIRP CMKKTIYENEGFREIKGYEY QLYVYASDKLFRADISEDYK TRGRKLLRFNGPVPPP

Data sets:
Data typeCount
15N chemical shifts102
1H chemical shifts604
coupling constants114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MNEI1

Entities:

Entity 1, MNEI 96 residues - Formula weight is not available

1   GLYGLUTRPGLUILEILEASPILEGLYPRO
2   PHETHRGLNASNLEUGLYLYSPHEALAVAL
3   ASPGLUGLUASNLYSILEGLYGLNTYRGLY
4   ARGLEUTHRPHEASNLYSVALILEARGPRO
5   CYSMETLYSLYSTHRILETYRGLUASNGLU
6   GLYPHEARGGLUILELYSGLYTYRGLUTYR
7   GLNLEUTYRVALTYRALASERASPLYSLEU
8   PHEARGALAASPILESERGLUASPTYRLYS
9   THRARGGLYARGLYSLEULEUARGPHEASN
10   GLYPROVALPROPROPRO

Samples:

sample_1: MNEI SWEET PROTEIN RELATED TO MONELLIN, [U-15N], 2 mM; phosphate buffer K 18.5 mM; H2O 90%; D2O 10%

sample_2: MNEI SWEET PROTEIN RELATED TO MONELLIN 2 mM; phosphate buffer K 18.5 mM; H2O 90%; D2O 10%

sample_3: MNEI SWEET PROTEIN RELATED TO MONELLIN 2 mM; phosphate buffer K 18.5 mM; D2O 100%

sample_cond_1: ionic strength: 18.5 mM; pH: 2.9; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
HSQCnot availablenot availablesample_cond_1
HNHBnot availablenot availablesample_cond_1
E-COSYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1

Software:

NMRPipe v1.7 - processing

DYANA v1.5 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 4222
PDB
GB AFF58925
PRF 761801B
SP P02882