BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4639

Title: NMR structure of the hRap1 Myb motif reveals a canonical three helix bundle lacking the positive surface charge typical of Myb DNA binding domains

Authors: Hanaoka, S; Nagadoi, A; Yoshimura, S; Aimoto, S; Li, B; de Lange, T; Nishimura, Y

Citation: Hanaoka, S; Nagadoi, A; Yoshimura, S; Aimoto, S; Li, B; de Lange, T; Nishimura, Y. "NMR structure of the hRap1 Myb motif reveals a canonical three helix bundle lacking the positive surface charge typical of Myb DNA binding domains"  J. Mol. Biol. 312, 167-175 (2001).

Assembly members:
TRF2-INTERACTING TELOMERIC RAP1 PROTEIN, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
TRF2-INTERACTING TELOMERIC RAP1 PROTEIN: GRIAFTDADDVAILTYVKEN ARSPSSVTGNALWKAMEKSS LTQHSWQSLKDRYLKHLRG

Data sets:
Data typeCount
1H chemical shifts379

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hRap1 Myb domain1

Entities:

Entity 1, hRap1 Myb domain 59 residues - Formula weight is not available

1   GLYARGILEALAPHETHRASPALAASPASP
2   VALALAILELEUTHRTYRVALLYSGLUASN
3   ALAARGSERPROSERSERVALTHRGLYASN
4   ALALEUTRPLYSALAMETGLULYSSERSER
5   LEUTHRGLNHISSERTRPGLNSERLEULYS
6   ASPARGTYRLEULYSHISLEUARGGLY

Samples:

sample_1: TRF2-INTERACTING TELOMERIC RAP1 PROTEIN 1.0 mM; potassium phosphate 100 mM; NaN3 1.0 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.5; temperature: 300 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H DQF-COSYsample_1not availablesample_cond_1
2D 1H-1H NOESYsample_1not availablesample_cond_1
2D 1H-1H TOCSYsample_1not availablesample_cond_1

Software:

NMRPipe v4.2.5 - processing

X-PLOR v3.851 - structure solution

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAA91317 BAE00469 BAE01995 BAE87910 BAE88172
GB AAF72711 AAH04465 AAH05841 AAH22428 AAH78171
REF NP_001267142 NP_061848 XP_001110856 XP_003780774 XP_003829596
SP Q4R4I0 Q9NYB0