BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4643

Title: The solution structure of sheep myeloid antimicrobial peptide (smap29) and its relationship to biological function   PubMed: 11856344

Authors: Waring, A.; Sawai, M.; McCray, P.; Robertson, A.; Lehrer, R.

Citation: Tack, B.; Sawai, M.; Kearny, W.; Robertson, A.; Sherman, M.; Wang, W.; Hong, T.; Boo, L.; Wu, H.; Waring, A.; Lehrer, R.. "SMAP-29 has two LPS-binding sites and a central hinge"  Eur. J. Biochem. 269, 1181-1189 (2002).

Assembly members:
MYELOID ANTIMICROBIAL PEPTIDE, polymer, 29 residues, Formula weight is not available

Natural source:   Common Name: sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:   Production method: .

Entity Sequences (FASTA):
MYELOID ANTIMICROBIAL PEPTIDE: RGLRRLGRKIAHGVKKYGPT VLRIIRIAG

Data sets:
Data typeCount
1H chemical shifts203
coupling constants8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MYELOID ANTIMICROBIAL PEPTIDE1

Entities:

Entity 1, MYELOID ANTIMICROBIAL PEPTIDE 29 residues - Formula weight is not available

1   ARGGLYLEUARGARGLEUGLYARGLYSILE
2   ALAHISGLYVALLYSLYSTYRGLYPROTHR
3   VALLEUARGILEILEARGILEALAGLY

Samples:

sample_1: MYELOID ANTIMICROBIAL PEPTIDE 1 mM; phosphate buffer 50 mM; H2O 60%; trifluoroethanol, [U-2H], 40%

sample_cond_1: pH: 5.94; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
TOCSYsample_1not availablesample_cond_1

Software:

DYANA v1.5 - structure solution

VNMR v4.3b - collection

VNMR v6.1b - data analysis, refinement

NMR spectrometers:

  • Varian UNITY 500 MHz

Related Database Links:

PDB
EMBL CAA63412 CAA63413
GB AAA85470 AAB49715
REF NP_001009406
SP P49928 P49929