BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4644

Title: Solution structures of two CCHC zinc fingers from the FOG family protein U-shaped that mediate protein-protein interactions   PubMed: 11080638

Authors: Liew, C.; Kowalski, K.; Fox, A.; Newton, A.; Sharpe, B.; Crossley, M.; Mackay, J.

Citation: Liew, C.; Kowalski, K.; Fox, A.; Newton, A.; Sharpe, B.; Crossley, M.; Mackay, J.. "Solution structures of two CCHC zinc fingers from the FOG family protein U-shaped that mediate protein-protein interactions"  Structure Fold Des. 8, 1157-1166 (2000).

Assembly members:
FIRST ZINC FINGER OF U-SHAPED, polymer, 36 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FIRST ZINC FINGER OF U-SHAPED: GSLLKPARFMCLPCGIAFSS PSTLEAHQAYYCSHRI

Data sets:
Data typeCount
1H chemical shifts215
15N chemical shifts32
coupling constants28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FIRST ZINC FINGER OF U-SHAPED1
2ZINC ION2

Entities:

Entity 1, FIRST ZINC FINGER OF U-SHAPED 36 residues - Formula weight is not available

1   GLYSERLEULEULYSPROALAARGPHEMET
2   CYSLEUPROCYSGLYILEALAPHESERSER
3   PROSERTHRLEUGLUALAHISGLNALATYR
4   TYRCYSSERHISARGILE

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: FIRST ZINC FINGER OF U-SHAPED 0.7 mM; H2O 95%; D2O 5%

sample_2: FIRST ZINC FINGER OF U-SHAPED, [U-15N], 0.7 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 5.5; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
HNHBnot availablenot availablesample_cond_1

Software:

XWINNMR v2.6 - collection

XEASY v1.2 - data analysis

DYANA v1.5 - structure solution

CNS v1.0 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAA72991
GB AAF51488 AAT94507 ACO56241 AGB92365 AGB92366
REF NP_001259828 NP_001259829 NP_001259830 NP_001259831 NP_476780
SP Q9VPQ6