BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4650

Title: Sheep prion protein synthetic peptide spanning helix 1 and b strand 2 (residues 142 to 166) shows b hairpin structure in solution   PubMed: 11577109

Authors: Kozin, S.; Bertho, G.; Mazur, A.; Rabesona, H.; Girault, J.-P.; Haertle, T.; Takahashi, M.; Debey, P.; Hui Bon Hoa, G.

Citation: Kozin, S.; Bertho, G.; Mazur, A.; Rabesona, H.; Girault, J.-P.; Haertle, T.; Takahashi, M.; Debey, P.; Hui Bon Hoa, G.. "Structural Duality of a Peptide from Sheep Prion Protein may be One of the Causes of Prion Conversion"  J. Biol. Chem. 276, 46364-46370 (2001).

Assembly members:
Major Prion Protein, polymer, 26 residues, Formula weight is not available

Natural source:   Common Name: Sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Major Prion Protein: GNDYEDRYYRENMYRYPNQV YYRPVC

Data sets:
Data typeCount
1H chemical shifts201
coupling constants19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SHEEP PRION PEPTIDE1

Entities:

Entity 1, SHEEP PRION PEPTIDE 26 residues - Formula weight is not available

1   GLYASNASPTYRGLUASPARGTYRTYRARG
2   GLUASNMETTYRARGTYRPROASNGLNVAL
3   TYRTYRARGPROVALCYS

Samples:

sample_1: Major Prion Protein 4.5 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 15 mM; pH: 6.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
1D, 2D DQF-COSYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
2D ROESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
PFG HSQCsample_1not availablesample_cond_1
PFG HMBCsample_1not availablesample_cond_1
2D 1H-13C HSQCsample_1not availablesample_cond_1
2D 1H-13C HMBCsample_1not availablesample_cond_1

Software:

xwinnmr v2.1 - collection

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 15394 15399 16328 16616 25250 25251
PDB
DBJ BAA07376 BAB69955 BAB69956 BAD51981 BAE91721
EMBL CAA04234 CAA04235 CAA04236 CAA04272 CAA04273
GB AAA68634 AAA68635 AAA68941 AAB03105 AAB50623
PRF 1921132A
REF NP_001009481 NP_001040617 NP_001075490 NP_001124544 NP_001137270
SP O18754 P23907 P40245 P40246 P40247
TPE CAJ43808 CAL59564