BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4661

Title: Solution structure of APAF-1 CARD

Authors: Zhou, Pei; Chou, James; Sanchez-Olea, Roberto; Yuan, Junying; Wagner, Gerhard

Citation: Zhou, Pei; Chou, James; Sanchez-Olea, Roberto; Yuan, Junying; Wagner, Gerhard. "Solution structure of Apaf-1 CARD and its interaction with caspase-9 CARD: A structural basis for specific adaptor/caspase interaction"  Proc. Natl. Acad. Sci. U.S.A. 96, 11265-11270 (1999).

Assembly members:
APOPTOTIC PROTEASE ACTIVATING FACTOR 1, polymer, 97 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
APOPTOTIC PROTEASE ACTIVATING FACTOR 1: MDAKARNCLLQHREALEKDI KTSYIMDHMISDGFLTISEE EKVRNEPTQQQRAAMLIKMI LKKDNDSYVSFYNALLHEGY KDLAALLHDGIPVVSSS

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts90
1H chemical shifts627
13C chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Apaf-1 CARD1

Entities:

Entity 1, Apaf-1 CARD 97 residues - Formula weight is not available

1   METASPALALYSALAARGASNCYSLEULEU
2   GLNHISARGGLUALALEUGLULYSASPILE
3   LYSTHRSERTYRILEMETASPHISMETILE
4   SERASPGLYPHELEUTHRILESERGLUGLU
5   GLULYSVALARGASNGLUPROTHRGLNGLN
6   GLNARGALAALAMETLEUILELYSMETILE
7   LEULYSLYSASPASNASPSERTYRVALSER
8   PHETYRASNALALEULEUHISGLUGLYTYR
9   LYSASPLEUALAALALEULEUHISASPGLY
10   ILEPROVALVALSERSERSER

Samples:

sample_1: APOPTOTIC PROTEASE ACTIVATING FACTOR 1, [U-15N], 0.5 – 1 mM; phosphate buffer 20 mM; NaCl 50 mM; DTT 5 mM

sample_2: APOPTOTIC PROTEASE ACTIVATING FACTOR 1, [U-13C], 1 mM; phosphate buffer 20 mM; NaCl 50 mM; DTT 5 mM

sample_3: APOPTOTIC PROTEASE ACTIVATING FACTOR 1 1 mM; phosphate buffer 20 mM; NaCl 50 mM; DTT 5 mM

sample_4: APOPTOTIC PROTEASE ACTIVATING FACTOR 1, [U-13C; U-15N; U-80% 2H], 1 mM; phosphate buffer 20 mM; NaCl 50 mM; DTT 5 mM

sample_cond_1: pH: 6.5; temperature: 300 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYnot availablenot availablesample_cond_1
3D_13C-separated_NOESYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
HMQC-Jnot availablenot availablesample_cond_1

Software:

DYANA v1.5 - Structure solution

X-PLOR v3.851 - refinement

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
DBJ BAA24843 BAC77343 BAJ20211
EMBL CAB55579 CAB55580 CAB55581 CAB55582 CAB55583
GB AAC51678 AAD34016 AAD38344 AAI36532 AAI36533
REF NP_001151 NP_037361 NP_863651 NP_863658 NP_863659
SP O14727