BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4663

Title: Rotamer Strain as a Determinant of Protein Structural Specificity   PubMed: 10631975

Authors: Lazar, G.; Johnson, E.; Desjarlais, J.; Handel, T.

Citation: Lazar, G.; Johnson, E.; Desjarlais, J.; Handel, T.. "Rotamer Strain as a Determinant of Protein Structural Specificity"  Protein Sci. 8, 2598-2610 (1999).

Assembly members:
PROTEIN (1D8 UBIQUITIN), polymer, 76 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PROTEIN (1D8 UBIQUITIN): MQLFVKTLTGKTLTVELEPS DTVENLKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN LQKESTIHLVLRLRGG

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts84
1H chemical shifts571

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11D8-UBIQUITIN1

Entities:

Entity 1, 1D8-UBIQUITIN 76 residues - Formula weight is not available

1   METGLNLEUPHEVALLYSTHRLEUTHRGLY
2   LYSTHRLEUTHRVALGLULEUGLUPROSER
3   ASPTHRVALGLUASNLEULYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   LEUGLNLYSGLUSERTHRILEHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_one: PROTEIN (1D8 UBIQUITIN), [U-13C; U-15N], 2 mM; sodium acetate D3 25 mM; sodium azide 0.02%

sample_conditions: ionic strength: 50 mM; pH: 5.80; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N NOESYsample_onenot availablesample_conditions
3D 13C NOESYsample_onenot availablesample_conditions
4D 13C/13C NOESYsample_onenot availablesample_conditions
2D JCCOCsample_onenot availablesample_conditions
2D JCNCsample_onenot availablesample_conditions
3D LRCCsample_onenot availablesample_conditions
3D HACAHB AND HNHAsample_onenot availablesample_conditions
HNHBsample_onenot availablesample_conditions
H/D EXCHANGE FOR H-BONDSsample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • BRUKER DMX 600 MHz

Related Database Links:

PDB
GB AHB33736