BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4664

Title: Sequence-Specific Resonance Assignments of Q83, a Lipocalin Highly Expressed in v-myc-Transformed Avian Fibroblasts

Authors: Kontaxis, Georg; Matt, Theresia; Schuler, Wolfgang; Krautler, Bernhard; Bister, Klaus; Konrat, Robert

Citation: Kontaxis, Georg; Matt, Theresia; Schuler, Wolfgang; Krautler, Bernhard; Bister, Klaus; Konrat, Robert. "Sequence-specific Resonance Assignments of Q83, a Lipocalin Highly Expressed in v-myc-transformed Avian Fibroblasts"  J. Biomol. NMR 17, 177-178 (2000).

Assembly members:
Q83, polymer, 157 residues, Formula weight is not available

Natural source:   Common Name: quail   Taxonomy ID: 9091   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Coturnix coturnix

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Q83: MTVPDRSEIAGKWYVVALAS NTEFFLREKDKMKMAMARIS FLGEDELKVSYAVPKPNGCR KWETTFKKTSDDGEVYYSEE AKKKVEVLDTDYKSYAVIYA TRVKDGRTLHMMRLYSRSPE VSPAATAIFRKLAGERNYTD EMVAMLPRQEECTVDEV

Data sets:
Data typeCount
1H chemical shifts493
13C chemical shifts419
15N chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Q831

Entities:

Entity 1, Q83 157 residues - Formula weight is not available

1   METTHRVALPROASPARGSERGLUILEALA
2   GLYLYSTRPTYRVALVALALALEUALASER
3   ASNTHRGLUPHEPHELEUARGGLULYSASP
4   LYSMETLYSMETALAMETALAARGILESER
5   PHELEUGLYGLUASPGLULEULYSVALSER
6   TYRALAVALPROLYSPROASNGLYCYSARG
7   LYSTRPGLUTHRTHRPHELYSLYSTHRSER
8   ASPASPGLYGLUVALTYRTYRSERGLUGLU
9   ALALYSLYSLYSVALGLUVALLEUASPTHR
10   ASPTYRLYSSERTYRALAVALILETYRALA
11   THRARGVALLYSASPGLYARGTHRLEUHIS
12   METMETARGLEUTYRSERARGSERPROGLU
13   VALSERPROALAALATHRALAILEPHEARG
14   LYSLEUALAGLYGLUARGASNTYRTHRASP
15   GLUMETVALALAMETLEUPROARGGLNGLU
16   GLUCYSTHRVALASPGLUVAL

Samples:

sample_1: Q83, [U-13C; U-15N], 3.0 mM; potassium phosphate 20 mM; potassium chloride 50 mM; dithiothreitol 0.5 mM

Ex-cond_1: pH: 6.4; temperature: 299 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
CBCA(CO)NNHsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
1H-15N TOCSY-HSQCsample_1not availableEx-cond_1
1H-15N NOESY-HSQCsample_1not availableEx-cond_1

Software:

NMRPipe - spectra processing

ANSIG - spectra assignment and evaluation

NMR spectrometers:

  • Varian Unity Plus 500 MHz

Related Database Links:

BMRB 16682 17577
PDB
GB AAF35894 AAK31634
SP Q9I9P7