BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4666

Title: Biotin carboxyl carrier domain of transcarboxylase (TC 1.3S)

Authors: Reddy, D.; Shenoy, B.; Carey, P.; Sonnichsen, F.

Citation: Reddy, D.; Shenoy, B.; Carey, P.; Sonnichsen, F.. "High Resolution Solution Structure of the 1.3S Subunit of Transcarboxylase from Propionibacterium shermanii"  Biochemistry 39, 2509-2516 (2000).

Assembly members:
TRANSCARBOXYLASE 1.3S SUBUNIT, polymer, 77 residues, 12367 Da.
NORBIOTIN, non-polymer, 230.284 Da.

Natural source:   Common Name: Propionibacterium Freudenreichii subsp shermanii   Taxonomy ID: 1752   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Propionibacterium Freudenreichii subsp shermanii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TRANSCARBOXYLASE 1.3S SUBUNIT: AGAGKAGEGEIPAPLAGTVS KILVKEGDTVKAGQTVLVLE AMKMETEINAPTDGKVEKVL VKERDAVQGGQGLIKIG

Data sets:
Data typeCount
15N chemical shifts80
1H chemical shifts545
13C chemical shifts247
coupling constants47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1transcarboxylase 1.3S subunit1
2biotin moiety2

Entities:

Entity 1, transcarboxylase 1.3S subunit 77 residues - 12367 Da.

1   ALAGLYALAGLYLYSALAGLYGLUGLYGLU
2   ILEPROALAPROLEUALAGLYTHRVALSER
3   LYSILELEUVALLYSGLUGLYASPTHRVAL
4   LYSALAGLYGLNTHRVALLEUVALLEUGLU
5   ALAMETLYSMETGLUTHRGLUILEASNALA
6   PROTHRASPGLYLYSVALGLULYSVALLEU
7   VALLYSGLUARGASPALAVALGLNGLYGLY
8   GLNGLYLEUILELYSILEGLY

Entity 2, biotin moiety - C9 H14 N2 O3 S - 230.284 Da.

1   SNR

Samples:

sample_1: TRANSCARBOXYLASE 1.3S SUBUNIT, [U-95%13C; U-90%15N], 1 – 2 mM

sample_2: TRANSCARBOXYLASE 1.3S SUBUNIT, [U-90% 15N], 1 – 2 mM

sample_cond_1: pH: 6.7; temperature: 293 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_cond_1

Software:

NMRPIPE vSGI6X.M4 - processing

PIPP v3.7.3 - data analysis

X-PLOR v3.81 - structure solution

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

BMRB 4667
PDB
EMBL CAD59402 CBL57376 CDP49157 CEG86413 CEG88429
GB AAA25674 AAA89090 AAA89092 AAA89094 AJQ91496
REF WP_013161729 WP_048769525
SP P02904