BMRB Entry 4677

Title:
Solution Structure of the Cysteine-rich Domain of the Escherichia coli Chaperone Protein DnaJ
Deposition date:
2000-03-02
Original release date:
2001-05-03
Authors:
Martinez-Yamout, Maria; Legge, Glen; Zhang, Ouwen; Wright, Peter; Dyson, Jane
Citation:

Citation: Martinez-Yamout, Maria; Legge, Glen; Zhang, Ouwen; Wright, Peter; Dyson, Jane. "Solution Structure of the Cysteine-rich Domain of the Escherichia coli Chaperone Protein DnaJ"  J. Mol. Biol. 300, 805-818 (2000).

Assembly members:

Assembly members:
cysteine-rich domain of DnaJ, polymer, 79 residues, 8518 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cysteine-rich domain of DnaJ: GVTKEIRIPTLEECDVCHGS GAKPGTQPQTCPTCHGSGQV QMRQGFFAVQQTCPHCQGRG TLIKDPCNKCHGHGRVERS

Data sets:
Data typeCount
13C chemical shifts300
15N chemical shifts81
1H chemical shifts482

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRD monomer1
2Zn12
3Zn22

Entities:

Entity 1, CRD monomer 79 residues - 8518 Da.

1   GLYVALTHRLYSGLUILEARGILEPROTHR
2   LEUGLUGLUCYSASPVALCYSHISGLYSER
3   GLYALALYSPROGLYTHRGLNPROGLNTHR
4   CYSPROTHRCYSHISGLYSERGLYGLNVAL
5   GLNMETARGGLNGLYPHEPHEALAVALGLN
6   GLNTHRCYSPROHISCYSGLNGLYARGGLY
7   THRLEUILELYSASPPROCYSASNLYSCYS
8   HISGLYHISGLYARGVALGLUARGSER

Entity 2, Zn1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: cysteine-rich domain of DnaJ, [U-95% 13C; U-90% 15N], 0.6 mM

ex-cond_1: pH: 7.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHsample_1not availableex-cond_1
HNCACBsample_1not availableex-cond_1
C(CO)NH-TOOCSYsample_1not availableex-cond_1
HNCOsample_1not availableex-cond_1
HCCH-TOCSYsample_1not availableex-cond_1
15N-edited NOESYsample_1not availableex-cond_1
15N-edited TOCSYsample_1not availableex-cond_1
13C-edited NOESYsample_1not availableex-cond_1
13C, 15N-edited NOESYsample_1not availableex-cond_1

Software:

Felix v97 - transform and plotting

NMRPipe - transform

NMRView - plotting and analysis

NMR spectrometers:

  • Brruker DMX 750 MHz
  • Brruker DRX 600 MHz
  • Brruker AMX 500 MHz

Related Database Links:

PDB
DBJ BAB33438 BAB96590 BAF79748 BAF79749 BAF79793
GenBank AAA00009 AAA23693 AAC73126 AAG54315 AAN41681
REF AP_000679 NP_285707 NP_308042 NP_414556 NP_705974
SWISS-PROT A7ZHA5 A7ZVV8 B1IRF9 P08622 Q0T8H5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks