BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4677

Title: Solution Structure of the Cysteine-rich Domain of the Escherichia coli Chaperone Protein DnaJ

Authors: Martinez-Yamout, Maria; Legge, Glen; Zhang, Ouwen; Wright, Peter; Dyson, Jane

Citation: Martinez-Yamout, Maria; Legge, Glen; Zhang, Ouwen; Wright, Peter; Dyson, Jane. "Solution Structure of the Cysteine-rich Domain of the Escherichia coli Chaperone Protein DnaJ"  J. Mol. Biol. 300, 805-818 (2000).

Assembly members:
cysteine-rich domain of DnaJ, polymer, 79 residues, 8518 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
cysteine-rich domain of DnaJ: GVTKEIRIPTLEECDVCHGS GAKPGTQPQTCPTCHGSGQV QMRQGFFAVQQTCPHCQGRG TLIKDPCNKCHGHGRVERS

Data sets:
Data typeCount
1H chemical shifts482
13C chemical shifts300
15N chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRD monomer1
2Zn12
3Zn22

Entities:

Entity 1, CRD monomer 79 residues - 8518 Da.

1   GLYVALTHRLYSGLUILEARGILEPROTHR
2   LEUGLUGLUCYSASPVALCYSHISGLYSER
3   GLYALALYSPROGLYTHRGLNPROGLNTHR
4   CYSPROTHRCYSHISGLYSERGLYGLNVAL
5   GLNMETARGGLNGLYPHEPHEALAVALGLN
6   GLNTHRCYSPROHISCYSGLNGLYARGGLY
7   THRLEUILELYSASPPROCYSASNLYSCYS
8   HISGLYHISGLYARGVALGLUARGSER

Entity 2, Zn1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: cysteine-rich domain of DnaJ, [U-95% 13C; U-90% 15N], 0.6 mM

ex-cond_1: pH: 7.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHsample_1not availableex-cond_1
HNCACBsample_1not availableex-cond_1
C(CO)NH-TOOCSYsample_1not availableex-cond_1
HNCOsample_1not availableex-cond_1
HCCH-TOCSYsample_1not availableex-cond_1
15N-edited NOESYsample_1not availableex-cond_1
15N-edited TOCSYsample_1not availableex-cond_1
13C-edited NOESYsample_1not availableex-cond_1
13C, 15N-edited NOESYsample_1not availableex-cond_1

Software:

Felix v97 - transform and plotting

NMRPipe - transform

NMRView - plotting and analysis

NMR spectrometers:

  • Brruker DMX 750 MHz
  • Brruker DRX 600 MHz
  • Brruker AMX 500 MHz

Related Database Links:

PDB
DBJ BAB33438 BAB96590 BAF79722 BAF79723 BAF79724
EMBL CAP74585 CAQ30532 CAQ87599 CAQ96906 CAR01382
GB AAA00009 AAA23693 AAC73126 AAG54315 AAN41681
REF NP_308042 NP_414556 NP_705974 WP_000240600 WP_000303366
SP A7ZHA5 A7ZVV8 B1IRF9 B1LFU5 B1XBE0