BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4682

Title: Backbone and side-chain 1H, 13C, and 15N Chemical Shift Assignments for holo-CRBP II   PubMed: 10884357

Authors: Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen

Citation: Lu, Jianyun; Lin, Chan-Lan; Tang, Changguo; Ponder, Jay; Kao, Jeff; Cistola, David; Li, Ellen. "Binding of retinol induces changes in rat cellular retinol-binding protein II conformation and backbone dynamics"  J. Mol. Biol. 300, 619-632 (2000).

Assembly members:
Holo cellular retinol-binding protein II, polymer, 134 residues, 286.452 Da.
RTL, non-polymer, 286.452 Da.

Natural source:   Common Name: norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Holo cellular retinol-binding protein II: MTKDQNGTWEMESNENFEGY MKALDIDFATRKIAVRLTQT KIIVQDGDNFKTKTNSTFRN YDLDFTVGVEFDEHTKGLDG RNVKTLVTWEGNTLVCVQKG EKENRGWKQWVEGDKLYLEL TCGDQVCRQVFKKK

Data sets:
Data typeCount
13C chemical shifts544
1H chemical shifts1496
15N chemical shifts304

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Holo CRBP II1
2RTL2

Entities:

Entity 1, Holo CRBP II 134 residues - 286.452 Da.

1   METTHRLYSASPGLNASNGLYTHRTRPGLU
2   METGLUSERASNGLUASNPHEGLUGLYTYR
3   METLYSALALEUASPILEASPPHEALATHR
4   ARGLYSILEALAVALARGLEUTHRGLNTHR
5   LYSILEILEVALGLNASPGLYASPASNPHE
6   LYSTHRLYSTHRASNSERTHRPHEARGASN
7   TYRASPLEUASPPHETHRVALGLYVALGLU
8   PHEASPGLUHISTHRLYSGLYLEUASPGLY
9   ARGASNVALLYSTHRLEUVALTHRTRPGLU
10   GLYASNTHRLEUVALCYSVALGLNLYSGLY
11   GLULYSGLUASNARGGLYTRPLYSGLNTRP
12   VALGLUGLYASPLYSLEUTYRLEUGLULEU
13   THRCYSGLYASPGLNVALCYSARGGLNVAL
14   PHELYSLYSLYS

Entity 2, RTL - C20 H30 O - 286.452 Da.

1   RTL

Samples:

sample_1: Holo cellular retinol-binding protein II, [U-99% 13C; U-99% 15N], 1.0 – 2.0 mM

sample_2: Holo cellular retinol-binding protein II 1.5 mM

sample_3: Holo cellular retinol-binding protein II, [U-99% 15N], 1.5 mM

Ex-con_1: pH: 7.4; temperature: 298 K; ionic strength: 0.081 M

Ex-con_2: pH: 6.5; temperature: 298 K; ionic strength: 0.079 M

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz
  • Varian UnityPlus 500 MHz

Related Database Links:

BMRB 4681
PDB
DBJ BAB22708
EMBL CAA52268
GB AAA42022 AAI25552 AAI25554 EDL20985 EDL77463
REF NP_033060 NP_036772 XP_006243669
SP P06768 Q08652