BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4697

Title: Structure of the fMet-tRNAfMet-binding domain of B. stearothermophilus initiation factor IF2

Authors: Meunier, Sylvie; Spurio, Roberto; Czisch, Michael; Wechselberger, Rainer; Guennuegues, Marc; Gualerzi, Claudio; Boelens, Rolf

Citation: Meunier, Sylvie; Spurio, Roberto; Czisch, Michael; Wechselberger, Rainer; Guennuegues, Marc; Gualerzi, Claudio; Boelens, Rolf. "Structure of the fMet-tRNA(fMet)-binding domain of B. stearothermophilus initiation factor IF2"  EMBO J. 19, 1918-1926 (2000).

Assembly members:
fMet-tRNA binding domain of initiation factor IF2, polymer, 112 residues, Formula weight is not available

Natural source:   Common Name: Bacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
fMet-tRNA binding domain of initiation factor IF2: EIEAAMKGMLDPEYEEKVIG QAEVRQTFKVSKVGTIAGCY VTDGKITRDSKVRLIRQGIV VYEGEIDSLKRYKDDVREVA QGYECGLTIKNFNDIKEGDV IEAYVMQEVARA

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts122
1H chemical shifts688
heteronuclear NOE values90
T1 relaxation values90
T2 relaxation values90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IF2 C-21

Entities:

Entity 1, IF2 C-2 112 residues - Formula weight is not available

1   GLUILEGLUALAALAMETLYSGLYMETLEU
2   ASPPROGLUTYRGLUGLULYSVALILEGLY
3   GLNALAGLUVALARGGLNTHRPHELYSVAL
4   SERLYSVALGLYTHRILEALAGLYCYSTYR
5   VALTHRASPGLYLYSILETHRARGASPSER
6   LYSVALARGLEUILEARGGLNGLYILEVAL
7   VALTYRGLUGLYGLUILEASPSERLEULYS
8   ARGTYRLYSASPASPVALARGGLUVALALA
9   GLNGLYTYRGLUCYSGLYLEUTHRILELYS
10   ASNPHEASNASPILELYSGLUGLYASPVAL
11   ILEGLUALATYRVALMETGLNGLUVALALA
12   ARGALA

Samples:

sample_1: fMet-tRNA binding domain of initiation factor IF2, [U-98% 15N], 1.5 mM

sample_2: fMet-tRNA binding domain of initiation factor IF2, [U-10% 13C; U-98% 15N], 1.5 mM

conditions: ionic strength: 0.200 M; pH: 5.2; temperature: 312 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableconditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAD75548 GAD12201 GAJ59418
EMBL CAA27987
GB ABO66491 ACX78663 ADI27260 ADU93636 AEV18768
REF WP_008878544 WP_011230763 WP_011887154 WP_013145729 WP_013523417
SP A4IMD7 P04766 Q5L0I8