BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4700

Title: Structure of Cdc42 bound to the GTPase Binding Domain of PAK

Authors: Morreale, Angela; Venkatesan, Meenakshi; Mott, Helen; Owen, Darerca; Nietlispach, Daniel; Lowe, Peter; Laue, Ernest

Citation: Morreale, Angela; Venkatesan, Meenakshi; Mott, Helen; Owen, Darerca; Nietlispach, Daniel; Lowe, Peter; Laue, Ernest. "Structure of Cdc42 bound to the GTPase Binding Domain of PAK"  Nat. Struct. Biol. 7, 384-388 (2000).

Assembly members:
Cdc42, polymer, 185 residues, 20430.40 Da.
p21 activated kinase, polymer, 46 residues, 5127.64 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Cdc42: MQTIKCVVVGDGAVGKTCLL ISYTTNKFPSEYVPTVFDNY AVTVMIGGEPYTLGLFDTAG LEDYDRLRPLSYPQTDVFLV CFSVVSPSSFENVKEKWVPE ITHHCPKTPFLLVGTQIDLR DDPSTIEKLAKNKQKPITPE TAEKLARDLKAVKYVECSAL TQKGLKNVFDEAILAALEPP EPKKX
p21 activated kinase: GSISLPSDFEHTIHVGFDAV TGEFTGMPEQWARLLQTSNI TKSEQK

Data sets:
Data typeCount
13C chemical shifts741
1H chemical shifts1550
15N chemical shifts224

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cdc421
2PAK2

Entities:

Entity 1, Cdc42 185 residues - 20430.40 Da.

1   METGLNTHRILELYSCYSVALVALVALGLY
2   ASPGLYALAVALGLYLYSTHRCYSLEULEU
3   ILESERTYRTHRTHRASNLYSPHEPROSER
4   GLUTYRVALPROTHRVALPHEASPASNTYR
5   ALAVALTHRVALMETILEGLYGLYGLUPRO
6   TYRTHRLEUGLYLEUPHEASPTHRALAGLY
7   LEUGLUASPTYRASPARGLEUARGPROLEU
8   SERTYRPROGLNTHRASPVALPHELEUVAL
9   CYSPHESERVALVALSERPROSERSERPHE
10   GLUASNVALLYSGLULYSTRPVALPROGLU
11   ILETHRHISHISCYSPROLYSTHRPROPHE
12   LEULEUVALGLYTHRGLNILEASPLEUARG
13   ASPASPPROSERTHRILEGLULYSLEUALA
14   LYSASNLYSGLNLYSPROILETHRPROGLU
15   THRALAGLULYSLEUALAARGASPLEULYS
16   ALAVALLYSTYRVALGLUCYSSERALALEU
17   THRGLNLYSGLYLEULYSASNVALPHEASP
18   GLUALAILELEUALAALALEUGLUPROPRO
19   GLUPROLYSLYSGNP

Entity 2, PAK 46 residues - 5127.64 Da.

1   GLYSERILESERLEUPROSERASPPHEGLU
2   HISTHRILEHISVALGLYPHEASPALAVAL
3   THRGLYGLUPHETHRGLYMETPROGLUGLN
4   TRPALAARGLEULEUGLNTHRSERASNILE
5   THRLYSSERGLUGLNLYS

Samples:

sample_1: Cdc42, [U-13C; U-15N], 0.7 – 1.0 mM; p21 activated kinase, [U-13C; U-15N], 0.7 – 1.0 mM

Ex-cond_1: pH: 5.5; temperature: 298 K; ionic strength: 55 mM

Experiments:

NameSampleSample stateSample conditions
15N-edited NOESysample_1not availableEx-cond_1
13C-edited NOEsysample_1not availableEx-cond_1
HCCH-COSYsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HBHA(CBCACO)NHsample_1not availableEx-cond_1
13C/15N-Xfiltered NOESYsample_1not availableEx-cond_1

Software:

AZARA v3.3 - DATA PROCESSING

ANSIG - ASSIGNMENT

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15424
PDB
DBJ BAB22563 BAC16312 BAC34669 BAC35825 BAE01909 BAC16311.1 BAC16312.1 BAF85787.1
EMBL CAA90215 CAB57325 CAB57326 CAB57327 CAB57328 CAF91245.1 CAN87991.1 CAN87992.1 CAF94511.1
GB AAA37410 AAA52494 AAA52592 AAB40051 AAC00027
REF NP_001003254 NP_001008027 NP_001017070 NP_001018130 NP_001034891 XP_001363384.1 XP_001363301.1 NP_035165.2 NP_001070366.1 XP_508657.2
SP P60766 P60952 P60953 Q007T2 Q2KJ93
TPG DAA32122
SWISS-PROT Q13153 O88643 Q08E52 P35465
PRF 2003404A
GenBank ACA13260.1 ACA13261.1 EAW75028.1 EDL16325.1 EDM18463.1