BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4701

Title: Tumor suppressor INK4: refinement of p16/INK4A structure and determination of p15/INK4B structure by comparative modeling and NMR data

Authors: Yuan, Chunhua; Selby, Thomas; Byeon, In-Ja; Tsai, Ming-Daw

Citation: Yuan, Chunhua; Selby, Thomas; Li, Junan; Byeon, In-Ja; Tsai, Ming-Daw. "Tumor suppressor INK4: refinement of p16/INK4A structure and determination of p15/INK4B structure by comparative modeling and NMR data"  Protein Sci. ., .-. (2000).

Assembly members:
p15, polymer, 136 residues, Formula weight is not available

Natural source:   Common Name: Mus musculus   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
p15: GSPGIHMLGGSSDAGLATAA ARGQVETVRQLLEAGADPNA LNRFGRRPIQVMMMGSAQVA ELLLLHGAEPNCADPATLTR PVHDAAREGFLDTLVVLHRA GARLDVCDAWGRLPVDLAEE QGHRDIARYLHAATGD

Data sets:
Data typeCount
1H chemical shifts745
13C chemical shifts260
15N chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p151

Entities:

Entity 1, p15 136 residues - Formula weight is not available

1   GLYSERPROGLYILEHISMETLEUGLYGLY
2   SERSERASPALAGLYLEUALATHRALAALA
3   ALAARGGLYGLNVALGLUTHRVALARGGLN
4   LEULEUGLUALAGLYALAASPPROASNALA
5   LEUASNARGPHEGLYARGARGPROILEGLN
6   VALMETMETMETGLYSERALAGLNVALALA
7   GLULEULEULEULEUHISGLYALAGLUPRO
8   ASNCYSALAASPPROALATHRLEUTHRARG
9   PROVALHISASPALAALAARGGLUGLYPHE
10   LEUASPTHRLEUVALVALLEUHISARGALA
11   GLYALAARGLEUASPVALCYSASPALATRP
12   GLYARGLEUPROVALASPLEUALAGLUGLU
13   GLNGLYHISARGASPILEALAARGTYRLEU
14   HISALAALATHRGLYASP

Samples:

sample_1: p15, [U-15N], 0.2 – 0.4 mM; HEPES 4 mM; DTT 1 mM; EDTA 5. uM; D2O 5%; H2O 95%

sample_2: p15, [U-15N; U-13C], 0.2 – 0.4 mM

sample_3: p15, [U-15N; U-13C], 0.2 – 0.4 mM

Ex-cond_1: pH: 7.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available

Software:

XWINNMR v2.0 - Data collection and data procssing

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAE25719
GB AAB35360 AAB39833 AAB94534 AAC14569 AAD00232
PIR I78845
REF NP_031696 NP_570825 XP_006974062
SP P55271 P55272