BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4716

Title: 1H, 15N, and 13C NMR Backbone Assignments and Secondary Structure of the C-terminal Recombinant Fragment of Auxilin Including the J-domain

Authors: Han, Chae; Gruschus, James; Greener, Tsvika; Greene, Lois; Ferretti, James; Eisenberg, Evan

Citation: Han, Chae; Gruschus, James; Greener, Tsvika; Greene, Lois; Ferretti, James; Eisenberg, Evan. "Letter to the Editor: 1H, 15N, and 13C NMR backbone assignments and secondary structure of the C-terminal recombinant fragment of auxilin including the J-domain"  J. Biomol. NMR 17, 281-282 (2000).

Assembly members:
auxilin, polymer, 182 residues, 20563 Da.

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
auxilin: GPLGSPEFSMPHSSPQNRPN YNVSFSSMPGGQNERGKAAA NLEGKQKAADFEDLLSGQGF NAHKDKKGPRTIAEMRKEEM AKEMDPEKLKILEWIEGKER NIRALLSTMHTVLWAGETKW KPVGMADLVTPEQVKKVYRK AVLVVHPDKATGQPYEQYAK MIFMELNDAWSEFENQGQKP LY

Data sets:
Data typeCount
1H chemical shifts651
13C chemical shifts361
15N chemical shifts154

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1auxilin1

Entities:

Entity 1, auxilin 182 residues - 20563 Da.

1   GLYPROLEUGLYSERPROGLUPHESERMET
2   PROHISSERSERPROGLNASNARGPROASN
3   TYRASNVALSERPHESERSERMETPROGLY
4   GLYGLNASNGLUARGGLYLYSALAALAALA
5   ASNLEUGLUGLYLYSGLNLYSALAALAASP
6   PHEGLUASPLEULEUSERGLYGLNGLYPHE
7   ASNALAHISLYSASPLYSLYSGLYPROARG
8   THRILEALAGLUMETARGLYSGLUGLUMET
9   ALALYSGLUMETASPPROGLULYSLEULYS
10   ILELEUGLUTRPILEGLUGLYLYSGLUARG
11   ASNILEARGALALEULEUSERTHRMETHIS
12   THRVALLEUTRPALAGLYGLUTHRLYSTRP
13   LYSPROVALGLYMETALAASPLEUVALTHR
14   PROGLUGLNVALLYSLYSVALTYRARGLYS
15   ALAVALLEUVALVALHISPROASPLYSALA
16   THRGLYGLNPROTYRGLUGLNTYRALALYS
17   METILEPHEMETGLULEUASNASPALATRP
18   SERGLUPHEGLUASNGLNGLYGLNLYSPRO
19   LEUTYR

Samples:

auxilin_1: auxilin1.0 – 2.0 mM

condition_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQC NOESYnot availablenot availablenot available
15N-HSQC TOCSYnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
CBCANHnot availablenot availablenot available

Software:

NMRPipe -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA32318 BAG11195 BAH12344
GB AAA79037 AAI09280 AAI09281 AIC59549 EAX06533
REF NP_001243793 NP_001243794 NP_055602 NP_777261 XP_001089936
SP O75061 Q27974
TPG DAA31275