BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4717

Title: 1H, 15N, 13C, and 13CO Assignments for ZipA

Authors: Moy, Franklin; Glasfeld, Elizabeth; Powers, Robert

Citation: Moy, Franklin; Glasfeld, Elizabeth; Powers, Robert. "Letter to the Editor: 1H, 15N, 13C, and 13CO assignments and secondary structure determination of ZipA*"  J. Biomol. NMR 17, 275-276 (2000).

Assembly members:
E. coli ZipA, polymer, 144 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
E. coli ZipA: MDKPKRKEAVIIMNVAAHHG SELNGELLLNSIQQAGFIFG DMNIYHRHLSPDGSGPALFS LANMVKPGTFDPEMKDFTTP GVTIFMQVPSYGDELQNFKL MLQSAQHIADEVGGVVLDDQ RRMMTPQKLREYQDIIREVK DANA

Data sets:
Data typeCount
1H chemical shifts925
13C chemical shifts611
15N chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ZipA1

Entities:

Entity 1, ZipA 144 residues - Formula weight is not available

1   METASPLYSPROLYSARGLYSGLUALAVAL
2   ILEILEMETASNVALALAALAHISHISGLY
3   SERGLULEUASNGLYGLULEULEULEUASN
4   SERILEGLNGLNALAGLYPHEILEPHEGLY
5   ASPMETASNILETYRHISARGHISLEUSER
6   PROASPGLYSERGLYPROALALEUPHESER
7   LEUALAASNMETVALLYSPROGLYTHRPHE
8   ASPPROGLUMETLYSASPPHETHRTHRPRO
9   GLYVALTHRILEPHEMETGLNVALPROSER
10   TYRGLYASPGLULEUGLNASNPHELYSLEU
11   METLEUGLNSERALAGLNHISILEALAASP
12   GLUVALGLYGLYVALVALLEUASPASPGLN
13   ARGARGMETMETTHRPROGLNLYSLEUARG
14   GLUTYRGLNASPILEILEARGGLUVALLYS
15   ASPALAASNALA

Samples:

sample_1: E. coli ZipA, [U-15N], 1.0 mM; potassium phosphate 50 mM; NaN3 2 mM; DTT, [U-2H], 50 mM; H2O 90%; D2O 10%

sample_2: E. coli ZipA, [U-13C; U-15N], 1.0 mM; potassium phosphate 50 mM; NaN3 2 mM; DTT, [U-2H], 50 mM; H2O 90%; D2O 10%

Ex-cond_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAA16284 BAB36707 BAG78227 BAI26665 BAI31697
EMBL CAP76884 CAQ32790 CAQ99310 CAR03874 CAR09031
GB AAB42061 AAC75465 AAG57531 AAN43974 AAN81396
REF NP_311311 NP_416907 NP_708267 WP_000314370 WP_000983142
SP A1ADS9 A7ZPL3 A8A2Q8 B1IX59 B1LMK6