BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4721

Title: Structure of the Central Core Domain of TFIIEbeta with a Novel Double-stranded DNA-binding Surface

Authors: Okuda, M.; Watanabe, Y.; Okamura, H.; Hanaoka, F.; Ohkuma, Y.; Nishimura, Y.

Citation: Okuda, M.; Watanabe, Y.; Okamura, H.; Hanaoka, F.; Ohkuma, Y.; Nishimura, Y.. "Structure of the Central Core Domain of TFIIEbeta with a Novel Double-stranded DNA-binding Surface"  EMBO J. 19, 1346-1356 (2000).

Assembly members:
general transcription factor TFIIE-BETA, polymer, 81 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
general transcription factor TFIIE-BETA: ALSGSSGYKFGVLAKIVNYM KTRHQRGDTHPLTLDEILDE TQHLDIGLKQKQWLMTEALV NNPKIEVIDGKYAFKPKYNV R

Data sets:
Data typeCount
1H chemical shifts601
13C chemical shifts256
15N chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TFIIE-BETA1

Entities:

Entity 1, TFIIE-BETA 81 residues - Formula weight is not available

1   ALALEUSERGLYSERSERGLYTYRLYSPHE
2   GLYVALLEUALALYSILEVALASNTYRMET
3   LYSTHRARGHISGLNARGGLYASPTHRHIS
4   PROLEUTHRLEUASPGLUILELEUASPGLU
5   THRGLNHISLEUASPILEGLYLEULYSGLN
6   LYSGLNTRPLEUMETTHRGLUALALEUVAL
7   ASNASNPROLYSILEGLUVALILEASPGLY
8   LYSTYRALAPHELYSPROLYSTYRASNVAL
9   ARG

Samples:

sample_1: general transcription factor TFIIE-BETA, [U-15N; U-13C], 1 – 2 mM; phosphate buffer 20 mM; NaCl 500 mM

sample_3: general transcription factor TFIIE-BETA1 – 2 mM; phosphate buffer 20 mM; NaCl 500 mM

sample_cond_1: pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D_13C-SEPARATED_NOESYnot availablenot availablenot available
3D_15N-SEPARATED_NOESYnot availablenot availablenot available
4D_13C-SEPARATED_NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HMQC-Jnot availablenot availablenot available

Software:

NMRPIPE v1.6 - PROCESSING

PIPP, CAPP, STAPP v3.9 - DATA ANALYSIS

EMBOSS v5 - STRUCTURE CALCULATION

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
DBJ BAE91442 BAG73195
EMBL CAA45069
GB AAB20414 AAG39077 AAH30572 AAV38582 AAX36090
PRF 1802389A
REF NP_001247856 NP_002086 XP_001168104 XP_002756966 XP_003269588
SP P29084