BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4738

Title: Determination by High Field NMR Spectroscopy of the Longitudinal Electron Relaxation Rate in Cu(II)Plastocyanin from Anabaena variablis.

Authors: Ma, Lixin; Led, Jens

Citation: Ma, Lixin; Led, Jens. "Determination by High Field NMR Spectroscopy of the Longitudinal Electron Relaxation Rate in Cu(II)Plastocyanin from Anabaena variablis."  J. Am. Chem. Soc. 122, 7823-7824 (2000).

Assembly members:
Plastocyanin from Anabena variabilis, polymer, 105 residues, 10500 Da.
COPPER (II) ION, non-polymer, 63.546 Da.
COPPER (I) ION, non-polymer, 63.546 Da.

Natural source:   Common Name: Anabaena variabilis   Taxonomy ID: 1172   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Anabaena variabilis

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
Plastocyanin from Anabena variabilis: ETYTVKLGSDKGLLVFEPAK LTIKPGDTVEFLNNKVPPHN VVFDAALNPAKSADLAKSLS HKQLLMSPGQSTSTTFPADA PAGEYTFYCEPHRGAGMVGK ITVAG

Data sets:
  • assigned_chemical_shifts
  • other_data_list
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1plastocyanin peptide1
2Cu(II)2
3Cu(I)3

Entities:

Entity 1, plastocyanin peptide 105 residues - 10500 Da.

1   GLUTHRTYRTHRVALLYSLEUGLYSERASP
2   LYSGLYLEULEUVALPHEGLUPROALALYS
3   LEUTHRILELYSPROGLYASPTHRVALGLU
4   PHELEUASNASNLYSVALPROPROHISASN
5   VALVALPHEASPALAALALEUASNPROALA
6   LYSSERALAASPLEUALALYSSERLEUSER
7   HISLYSGLNLEULEUMETSERPROGLYGLN
8   SERTHRSERTHRTHRPHEPROALAASPALA
9   PROALAGLYGLUTYRTHRPHETYRCYSGLU
10   PROHISARGGLYALAGLYMETVALGLYLYS
11   ILETHRVALALAGLY

Entity 2, Cu(II) - Cu - 63.546 Da.

1   CU

Entity 3, Cu(I) - Cu - 63.546 Da.

1   CU1

Samples:

sample_one: Plastocyanin from Anabena variabilis 2.9 mM; COPPER (I) ION 2.9 mM

sample_two: Plastocyanin from Anabena variabilis 2.9 mM; COPPER (I) ION 2.38 mM; COPPER (II) ION 0.52 mM

sample_three: Plastocyanin from Anabena variabilis 2.9 mM; COPPER (I) ION 2.45 mM; COPPER (II) ION 0.45 mM

cond_set_1: pH: 7.50; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
unknownnot availableisotropiccond_set_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
DBJ BAB77782
EMBL CAA05338 CAA32527
GB AAA59364 ABA22358
PIR CUAI S06999
REF WP_010994435
SP O52830 P0C178 P46444 Q3M9H8