BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4740

Title: Solution Structure of a 8.3 kDa Protein (gene MTH1184) from Methanobacterium thermoautotrophicum   PubMed: 11017201

Authors: Kozlov, G.; Ekiel, I.; Gehring, K.

Citation: Christendat, D.; Yee, A.; Dharamsi, A.; Kluger, Y.; Savchenko, A.; Cort, J.; Booth, V.; Mackereth, C.; Saridakis, V.; Ekiel, I.; Kozlov, G.; Maxwell, K.; Wu, N.; Mcintosh, L.; Gehring, K.; Kennedy, M.; Davidson, A.; Pai, E.; Gerstein, M.; Edwards, A.; Arrowsmith, C.. "Structural proteomics of an archaeon"  Nat. Struct. Biol. 7, 903-909 (2000).

Assembly members:
8.3 KDA PROTEIN (GENE MTH1184), polymer, 71 residues, Formula weight is not available

Natural source:   Common Name: Bacteria   Taxonomy ID: 2166   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
8.3 KDA PROTEIN (GENE MTH1184): MYIIFRCDCGRALYSREGAK TRKCVCGRTVNVKDRRIFGR ADDFEEASELVRKLQEEKYG SCHFTNPSKRE

Data sets:
Data typeCount
1H chemical shifts331
13C chemical shifts127
15N chemical shifts68
coupling constants60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
18.3 KDA PROTEIN (GENE MTH1184)1

Entities:

Entity 1, 8.3 KDA PROTEIN (GENE MTH1184) 71 residues - Formula weight is not available

1   METTYRILEILEPHEARGCYSASPCYSGLY
2   ARGALALEUTYRSERARGGLUGLYALALYS
3   THRARGLYSCYSVALCYSGLYARGTHRVAL
4   ASNVALLYSASPARGARGILEPHEGLYARG
5   ALAASPASPPHEGLUGLUALASERGLULEU
6   VALARGLYSLEUGLNGLUGLULYSTYRGLY
7   SERCYSHISPHETHRASNPROSERLYSARG
8   GLU

Samples:

sample_1: 8.3 KDA PROTEIN (GENE MTH1184), [U-13C; U-15N], 1 – 2 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM

sample_2: 8.3 KDA PROTEIN (GENE MTH1184), [U-13C], 1 – 2 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM

sample_3: 8.3 KDA PROTEIN (GENE MTH1184), [U-15N], 1 – 2 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM

sample_4: 8.3 KDA PROTEIN (GENE MTH1184)1 – 2 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM

sample_cond_1: pH: 6.3; temperature: 305 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
3D 13C-SEPARATED NOESYnot availablenot availablenot available
3D 15N-SEPARATED NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

XWINNMR v2.1 - COLLECTION

GIFA vV.4 - PROCESSING

XEASY v1.3.13 - DATA ANALYSIS

CNS v0.5 - STRUCTURE SOLUTION

ARIA v0.1 - STRUCTURE SOLUTION

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAM70325
GB AAB85673
REF WP_010876808 WP_048175749
SP O27252