BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4745

Title: Solution Conformation of a Bulged Adenosine in an RNA Duplex by Relaxation Matrix Refinement

Authors: Thiviyanathan, Varatharasa; Guliaev, Anton; Leontis, Neocles; Gorenstein, David

Citation: Thiviyanathan, Varatharasa; Guliaev, Anton; Leontis, Neocles; Gorenstein, David. "Solution Conformation of a Bulged Adenosine in an RNA Duplex by Relaxation Matrix Refinement"  J. Mol. Biol. 300, 1143-1154 (2000).

Assembly members:
RNA, polymer, 13 residues, Formula weight is not available
RNA, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
RNA: GGCAGAGUGCCGC
RNA: GCGGCACCUGCC

Data sets:
Data typeCount
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1bulged RNA, duplex 11
2bulged RNA, duplex 22

Entities:

Entity 1, bulged RNA, duplex 1 13 residues - Formula weight is not available

1   GGCAGAGUGC
2   CGC

Entity 2, bulged RNA, duplex 2 12 residues - Formula weight is not available

1   GCGGCACCUG
2   CC

Samples:

sample_1: RNA 1.0 mM; RNA 1.0 mM

conditions: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian VXR 500 MHz
  • Varian UnityPlus 600 MHz

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