BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4751

Title: Assignment of 1H and 15N resonances of mouse lysozyme

Authors: Obita, Takayuki; Ueda, Tadashi; Tanaka, Yoshitsugu; Hashimoto, Yoshio; Imoto, Taiji

Citation: Obita, Takayuki; Ueda, Tadashi; Tanaka, Yoshitsugu; Hashimoto, Yoshio; Imoto, Taiji. "Letter to the Editor: Assignment of 1H and 15N resonances of mouse lysozyme M"  J. Biomol. NMR 18, 361-362 (2000).

Assembly members:
mouse lysozyme, polymer, 130 residues, 14820 Da.

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
mouse lysozyme: KVYERCEFARTLKRNGMAGY YGVSLADWVCLAQHESNYNT RATNYNRGDQSTDYGIFQIN SRYWCNDGKTPRAVNACGIN CSALLQDDITAAIQCAKRVV RDPQGIRAWVAWRAHCQNRD LSQYIRNCGV

Data sets:
Data typeCount
1H chemical shifts709
15N chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse lysozyme1

Entities:

Entity 1, mouse lysozyme 130 residues - 14820 Da.

1   LYSVALTYRGLUARGCYSGLUPHEALAARG
2   THRLEULYSARGASNGLYMETALAGLYTYR
3   TYRGLYVALSERLEUALAASPTRPVALCYS
4   LEUALAGLNHISGLUSERASNTYRASNTHR
5   ARGALATHRASNTYRASNARGGLYASPGLN
6   SERTHRASPTYRGLYILEPHEGLNILEASN
7   SERARGTYRTRPCYSASNASPGLYLYSTHR
8   PROARGALAVALASNALACYSGLYILEASN
9   CYSSERALALEULEUGLNASPASPILETHR
10   ALAALAILEGLNCYSALALYSARGVALVAL
11   ARGASPPROGLNGLYILEARGALATRPVAL
12   ALATRPARGALAHISCYSGLNASNARGASP
13   LEUSERGLNTYRILEARGASNCYSGLYVAL

Samples:

sample_1: mouse lysozyme, [U-15N], 1.0 mM

sample_conditions_1: pH: 3.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
15N-edited NOESYnot availablenot availablenot available
15N-edited TOCSYnot availablenot availablenot available
1H NOESYnot availablenot availablenot available
1H TOCSYnot availablenot availablenot available
1H COSYnot availablenot availablenot available

Software:

NMR-PIPE - raw spectral data transformations

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAE28595 BAE30022 BAE31835 BAE32025 BAE34954
GB AAA39473 AAH02069 AAH54463 EDL21835
REF NP_059068
SP P08905
TPE CDM98779