BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4752

Title: 1H,13C,15N chemical shift assignments for the DNA binding domain of gpNu1

Authors: De Beer, Tonny; Catalano, Carlos

Citation: de Beer, Tonny; Ortega, Marcos; Berton, Nancy; Yang, Qin; Overduin, Michael; Catalano, Carlos. "Letter to the Editor: Assignment of the 1H, 13C, and 15N resonances of the DNA binding domain of gpNu1, a genome packaging protein from bacteriophage l"  J. Biomol. NMR 18, 69-70 (2000).

Assembly members:
gpnu1 DNA binding domain, polymer, 68 residues, 7808.2 Da.

Natural source:   Common Name: not available   Taxonomy ID: 10710   Superkingdom: Viruses   Kingdom: not available   Genus/species: Bacteriophage lambda

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
gpnu1 DNA binding domain: MEVNKKQLADIFGASIRTIQ NWQEQGMPVLRGGGKGNEVL YDSAAVIKWYAERDAEIENE KLRREVEE

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts494
13C chemical shifts309
15N chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gpnu1 E68 A1
2gpnu1 E68 B1

Entities:

Entity 1, gpnu1 E68 A 68 residues - 7808.2 Da.

1   METGLUVALASNLYSLYSGLNLEUALAASP
2   ILEPHEGLYALASERILEARGTHRILEGLN
3   ASNTRPGLNGLUGLNGLYMETPROVALLEU
4   ARGGLYGLYGLYLYSGLYASNGLUVALLEU
5   TYRASPSERALAALAVALILELYSTRPTYR
6   ALAGLUARGASPALAGLUILEGLUASNGLU
7   LYSLEUARGARGGLUVALGLUGLU

Samples:

sample_1: gpnu1 DNA binding domain0.5 – 1.0 mM; Tris, [U-2H], 20 mM

sample_2: gpnu1 DNA binding domain, [U-98% 15N], 1.0 mM; Tris, [U-2H], 20 mM

sample_3: gpnu1 DNA binding domain, [U-98% 13C; U-98% 15N], 1.0 mM

Ex-cond_H20: pH: 7.2; temperature: 298 K; pressure: 0.2 atm

Ex-cond_D2O: pH*: 6.8; temperature: 298 K; ionic strength: 0.2 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H NOESYnot availablenot availablenot available
2D 1H TOCSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-15N HSQC-NOESYnot availablenot availablenot available
1H-15N HMQC-NOESY-HMQCnot availablenot availablenot available
1H-15N HSQC-DIPSInot availablenot availablenot available
1H-15N HNHAnot availablenot availablenot available
1H-13C HSQCsnot availablenot availablenot available
3D 1H-13C HMQC-NOESYnot availablenot availablenot available
4D 1H-13C HMQC-NOESYnot availablenot availablenot available
2D (HB)CB(CGCD)HDnot availablenot availablenot available
2D HCCH-TOCSYnot availablenot availablenot available
CBCA(CO)NNHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
C-TOCSY-(CO)NNHnot availablenot availablenot available
H(C-TOCSY)-(CO)NNHnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

nmrPipe - data processing

Pipp - data analysis

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAA15252 BAB35052 BAE50804 BAE76336 BAG76117
EMBL CAP75662 CAQ31034 CAQ31247 CAQ97646 CAQ98446
GB AAA96533 AAC73661 AAG55979 AAN81626 AAZ89072
REF NP_040580 NP_287367 NP_309656 NP_415092 NP_755056
SP P03707 P31061 P31062