BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4759

Title: Backbone 1H, 15N and 13Calpha assigned chemical shifts for reduced Escherichia coli cytochrome b562

Authors: Assfalg, Michael; Banci, Lucia; Bertini, Ivano

Citation: Assfalg, Michael; Banci, Lucia; Bertini, Ivano. "Backbone 1H, 15N and 13Calpha assigned chemical shifts for reduced Escherichia coli cytochrome b56"  The BMRB entry is the only known published source for the data. (2000).

Assembly members:
cytochrome b562, polymer, 106 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
cytochrome b562: ADLEDNMETLNDNLKVIEKA DNAAQVKDALTKMRAAALDA QKATPPKLEDKSPDSPEMKD FRHGFDILVGQIDDALKLAN EGKVKEAQAAAEQLKTTRNA YHQKYR

Data sets:
Data typeCount
13C chemical shifts92
15N chemical shifts89
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome b5621
2Fe-heme2

Entities:

Entity 1, cytochrome b562 106 residues - Formula weight is not available

1   ALAASPLEUGLUASPASNMETGLUTHRLEU
2   ASNASPASNLEULYSVALILEGLULYSALA
3   ASPASNALAALAGLNVALLYSASPALALEU
4   THRLYSMETARGALAALAALALEUASPALA
5   GLNLYSALATHRPROPROLYSLEUGLUASP
6   LYSSERPROASPSERPROGLUMETLYSASP
7   PHEARGHISGLYPHEASPILELEUVALGLY
8   GLNILEASPASPALALEULYSLEUALAASN
9   GLUGLYLYSVALLYSGLUALAGLNALAALA
10   ALAGLUGLNLEULYSTHRTHRARGASNALA
11   TYRHISGLNLYSTYRARG

Entity 2, Fe-heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: cytochrome b562, [U-13C; U-15N], 2.0 mM

Ex-cond_1: pH: 4.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availableEx-cond_1
1H-15N HMQC-NOESYsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HNHAsample_1not availableEx-cond_1

Software:

UXNMR -

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 1672 4767
PDB
DBJ BAB38636 BAG80065 BAI28542 BAI33713 BAI38842
EMBL CAA47706 CAP78754 CAQ34585 CAQ91733 CAR01210
GB AAA97133 AAB20782 AAG59433 AAN45672 AAN83756
PIR E86121 E91280
REF NP_290867 NP_313240 NP_709965 NP_757182 WP_000112424
SP P0ABE7 P0ABE8 Q8CVG7