BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4771

Title: Assignment of the 1H, 15N and 13C resonances of the C-terminal domain of the TolA protein of Escherichia coli, involved in the cell envelope integrity

Authors: Deprez, Christophe; Blanchard, Laurence; Simorre, Jean-Pierre; Gavioli, Marthe; Guerlesquin, Francoise; Lazdunski, Claude; Lloubes, Roland; Marion, Dominique

Citation: Deprez, Christophe; Blanchard, Laurence; Simorre, Jean-Pierre; Gavioli, Marthe; Guerlesquin, Francoise; Lazdunski, Claude; Lloubes, Roland; Marion, Dominique. "Letter to the Editor: Assignment of the 1H, 15N and 13C resonances of the C-terminal domain of the TolA protein of Escherichia coli, involved in cell envelope integrity"  J. Biomol. NMR 18, 179-180 (2000).

Assembly members:
TolA, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TolA: AEFGNTKNNGASGADINNYA GQIKSAIESKFYDASSYAGK TCTLRIKLAPDGMLLDIKPE GGDPALCQAALAAAKLAKIP KPPSQAVYEVFKNAPLDFKP HHHHHH

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts597
13C chemical shifts390
15N chemical shifts91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tola31

Entities:

Entity 1, tola3 106 residues - Formula weight is not available

1   ALAGLUPHEGLYASNTHRLYSASNASNGLY
2   ALASERGLYALAASPILEASNASNTYRALA
3   GLYGLNILELYSSERALAILEGLUSERLYS
4   PHETYRASPALASERSERTYRALAGLYLYS
5   THRCYSTHRLEUARGILELYSLEUALAPRO
6   ASPGLYMETLEULEUASPILELYSPROGLU
7   GLYGLYASPPROALALEUCYSGLNALAALA
8   LEUALAALAALALYSLEUALALYSILEPRO
9   LYSPROPROSERGLNALAVALTYRGLUVAL
10   PHELYSASNALAPROLEUASPPHELYSPRO
11   HISHISHISHISHISHIS

Samples:

sample: TolA, [U-15N], 0.5 mM

condition: pH: 6.8; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
not availablesamplenot availablecondition

Software:

FELIX v2000 - Data processing and visualization

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

REF NP_706495 NP_308801 NP_415267 AP_001377 NP_286467
SWISS-PROT P19934
GenBank AAN42202 AAN79291 AAC73833 AAG55075 AAA24683
DBJ BAB34197 BAA35405
PDB
BMRB 6258